TABLE 1

Primers used to generate Mid1 CP mutant expression constructs

TargetPrimerSequence
Mid1 CP1x2xMid1-CP1x-RCCATTGATACCGCGGCTGAGCGAT
Mid1-CP2x-FGCATCAGGGCCGCGAAACGC
Mid1 CP1xMid1-CP1x-RCCATTGATACCGCGGCTGAGCGAT
Mid1-CP2-FGCATCAGGTGCCCGAAAC
Mid1 CP2xMid1-CP1-RCCATTGATACCGGGCATGAG
Mid1-CP2x-FGCATCAGGGCCGCGAAACGC
Mid1 CPwtMid1_NotI_FATAGCGGCCGCATGCCAGCGAGAGAGGTGTATTTCAAAAGG
Mid1_MfeI_RGCTCAATTGCTATCCGTTACACCATCTATTTCCCCAGCGAT