TABLE 3

Summary of the phenotypes observed for tetR-SEC6 and tetR-SEC15 strains

PhenotypetetR-SEC6tetR-SEC15
Stationary phaseaExponential phase
ViabilityViable up to 27 hViable up to 5 h
Secretion
    SapsDecreasedDecreased
    LipasesDecreasedDecreased
Accumulation of vesiclesDecreased (1 or 2 branches)Decreased (1 or 2 branches)Increased (3 or 4 branches)
AdherenceNot affectedDecreasedDecreased
Susceptibility to cell wall-degradative enzymes
    ZymolyaseResistantSensitiveSensitive
    ChitinaseResistantSensitiveSensitive
Mlc1-GFP localizationNormalNormalMislocalized
Spa2-GFP localizationNormalNormalMislocalized
Exo70-GFP localizationNormalNormalMislocalized
  • a Data from reference 6.