TABLE 1

Candida albicans strains used in this study

Strain nameParentGenotypeSource
THE1CAI8ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 SEC15/SEC1513
THE1-CIp10THE1ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 RPS1/RPS1::URA3 SEC6/SEC6 SEC15/SEC1512
SEC15Δ/+THE1ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 SEC15/sec15Δ::dpl200-URA3-dpl200This study
SEC15Δ/+ FOASEC15Δ/+ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 SEC15/sec15Δ::dpl200This study
tetR-SEC15SEC15 Δ/+ FOAade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 URA3-tetO-SEC15/sec15Δ::dpl200This study
tetR-SEC6SEC6 Δ/+ FOAade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 URA3-tetO-SEC6/sec6Δ::dpl2006
T-MLC1gfpTHE1-CIp10ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 RP10/RP10::URA3 MLC1/MLC1-GFP::NAT110
tSEC15-MLC1gfptetR-SEC15ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 tetR-URA3-SEC15/sec15Δ::dpl200 MLC1/MLC1-GFP::NAT1This study
tSEC6-MLC1gfptetR-SEC6ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 tetO-URA3-SEC6/sec6Δ::dpl200 MLC1/MLC1-GFP::NAT1This study
T-EXO70gfpTHE1-CIp10ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 RP10/RP10::URA3 EXO70/EXO70-GFP::NAT1This study
tSEC15-EXO70gfptetR-SEC15ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetR-ScHAP4AD-3 × HA-ADE2 tetO-URA3-SEC15/sec15Δ::dpl200 EXO70/EXO70-GFP::NAT1This study
tSEC6-EXO70gfptetR-SEC6ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 tetR-URA3-SEC6/sec6Δ::dpl200 EXO70/EXO70-GFP::NAT1This study
T-SPA2gfpTHE1-CIp10ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 RP10/RP10::URA3 SPA2/SPA2-GFP::NAT1This study
tSEC15-SPA2gfptetR-SEC15ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 tetR-URA3-SEC15/sec15Δ::dpl200 SPA2/SPA2-GFP::NAT1This study
tSEC6-SPA2gfptSEC6ade2::hisG/ade2::hisG ura3::imm434/ura3::imm434 ENO1/eno1::ENO1-tetO-ScHAP4AD-3 × HA-ADE2 tetR-URA3-SEC6/sec6Δ::dpl200 SPA2/SPA2-GFP::NAT1This study