TABLE 2

Mediator complex P sites

UniProt IDGene IDP site(s)a as detected by:
Large-scale phosphoproteomicsIn vitro Cdk8 kinase assay (ATP dependent)In vitro Cdk8 kinase assay (ATP independent)
Q5AHH0Med1S445, S446S445, S446
Q59Q94Med3S117, T125, T133T140S117, S181, S183, T119, T121, T125, T132, T133
Q59U73Med4S9, S13, S17, S33, S322, T36, T37, Y321S9, S33, T36, T37S9, S13
Q59KC0Med5S252S252
Q5A2Z1Med6
Q5AEN6Med7S285
Q59TD3Med8T37
Q5A0D0Med9S102T109
Q5A3K2Med10S170
Q59S43Med11S132, S142, T140, T144, T149S120, S132, S142, T140
Q59MN9Med12S594, S1033, S1579, S1680, S1682, S1713, T1039S63
Q5AG31Med14S385, T395S14, S385, S678, S763, S1170
Q5A757Med15S898, S939, S948, S1035, S1043, S1046, S1084, S1097, S1110, T903, T906, T913, T914, T937, T943, T1042S898, S900, S1082, S1084, T906, T913, T1030S898, S900, S948, S1043, S1082, S1083, S1084, T977, T1030, T1042
Q59PP6Med16S380
Q5AHZ7Med17S43, S73, T70
Q59X40Med18S207, T162
Med19
C4YHG8Med20
Q5ACU4Med21S79, S83S79S79, S85, S87
Q5A917Med22
Q59P87Med31
Q5ALX5Ssn2 (Med13)S399, S478, S493, S605, S615, S650, S652, S840, T476, T494, T774, T1449S493, T476, T774, T1449S348, S605, S833, S1343, S1345
Q5AHK2Ssn3 (Cdk8)S209S209
Q5A4H9Ssn8
TloS39T38
  • a P sites detected in the large-scale phosphoproteomics data set and the ATP-dependent in vitro Cdk8 kinase assay are in bold, and P sites detected in the large-scale phosphoproteomics data set and the ATP-independent in vitro Cdk8 kinase assay are in italics.