TABLE 1

Plaque assay of CRISPR-transfected parasites

sgRNAExpt% GFP-positive parasitesNo. of SNFr plaques at indicated no. of parasites/well% efficiency of SNFr at indicated no. of parasites/wellaAvg
1 × 1052 × 1042 × 1031 × 1052 × 1042 × 103
sgRNA-C6113.730158192.22.16.93.7
211.6587612652.611.26.2
sgRNA-UPRT150000000
29.30000000
  • a Calculated as no. of plaques/(no. of seeded parasites × percent GFP positive).