TABLE 2

Plaque assay of CRISPR-transfected parasitesa

sgRNANo. of SNFr plaques at indicated no. of parasites/well% SNFrbNo. of integrants/no. of clones tested
1 × 1041 × 103
sgRNA-C6Lysed898.99/11
sgRNA-C1Lysed646.47/11
sgRNA-UPRT000
  • a A DHFR* selection cassette was used in all cases.

  • b Calculated as no. of plaques/no. of seeded parasites.