TABLE 1

Apparent kinetic parameters for EhACD in each direction of the reactiona

Reaction direction and varied substrateKm (mM)kcat (s−1)kcat/Km (s−1 mM−1)
Acetate-forming direction
    Acetyl-CoA0.04 ± 0.001110 ± 1.62,600 ± 370
    Phosphate1.8 ± 0.18120 ± 7.865 ± 2.3
    ADP1.6 ± 0.15140 ± 1.289 ± 7.9
    GDP1.9 ± 0.14170 ± 5.290 ± 8.3
    Propionyl-CoA0.032 ± 0.00221 ± 0.6650 ± 63
    Phosphate1.5 ± 0.0918 ± 0.612 ± 0.5
    ADP0.71 ± 0.0924 ± 0.334 ± 3.4
Acetyl-CoA-forming direction
    Acetate14 ± 0.6240 ± 2.716 ± 0.5
    CoA0.20 ± 0.01220 ± 4.31,100 ± 57
    ATP12 ± 0.4320 ± 4.427 ± 0.7
    GTP10 ± 0.1180 ± 1.618 ± 0.1
    Propionate29 ± 1.4190 ± 1.56.3 ± 0.3
    CoA1.6 ± 0.05260 ± 2.0160 ± 4.3
    ATP7.2 ± 0.7240 ± 7.133 ± 2.2
  • a Values are means ± SD.