TABLE 2

Comparison of Km values for characterized ACD I enzymes

SubstrateKm (μM)
Archaeal ACDs (range)Bacterial ACDfGiardia ACDgEhACD
Acetyl-CoA10a to 410b372040
Phosphate100d to 1,300b,c1,0001,5901,800
ADP7a to 150d912301,600
Acetate340a to 1,700b900NDh14,000
CoA5.6e to 60b24ND200
ATP56e to 477d570ND12,000
  • a Reported Km for Archaeoglobus fulgidus (18).

  • b Reported Km for Haloarcula marismortui (16).

  • c Reported Km for Pyrobaculum aerophilum (16).

  • d Reported Km for Pyrococcus furiosus (12).

  • e Reported Km for Thermococcus kodakarensis (19).

  • f Reported Km for Chloroflexus aurantiacus (20).

  • g Reported Km for Giardia lamblia (10).

  • h ND, no activity detected in the direction of acetyl-CoA formation.