TABLE 1

Strains used or constructed in this study

StrainGenotypeReference
S. cerevisiae BW31aMATa leu2-3/122 ura3-1 trp1-1 his3-11/15 ade2-1 can1-100 GAL SUC2 mal10 ena1-4Δ::HIS3 nha1::LEU226
Z. rouxii
    CBS 732TWild typeCBS strain database
    UL4ura319
    F1ura3 Zrffz1Δ::loxPThis study
    F2ura3 Zrffz2Δ::loxPThis study
    F1F2ura3 Zrffz1Δ::loxP Zrffz2Δ::loxPThis study
    S1ura3 Zrfsy1Δ::loxP17
    F1F2S1ura3 Zrffz1Δ::loxP Zrffz2Δ::loxP Zrfsy1Δ::loxPThis study
    F1F2xura3 Zrffz1Δ::loxP Zrffz2Δ::loxPThis study
    F1F2(pZEU)F1F2 + pZEU; Zrffz1Δ::loxP Zrffz2Δ::loxPThis study
    F1F2(pZEU_ZrFFZ1)F1F2 + pZEU_ZrFFZ1; Zrffz2Δ::loxPThis study
    F1F2(pZCA_ZrFFZ1)F1F2 + pZCA_ZrFFZ1; Zrffz2Δ::loxPThis study
    F1F2(pZEU_ZrFFZ2)F1F2 + pZEU_ZrFFZ2; Zrffz1Δ::loxPThis study
    F1F2(pZGFP_ZrFFZ2)F1F2 + pZGFP_ZrFFZ2; Zrffz1Δ::loxPThis study
    F1F2(pZCA_ZrFFZ2)F1F2 + pZCA_ZrFFZ2; Zrffz1Δ::loxPThis study