TABLE 1

List of oligonucleotides used in experiments

OligonucleotideSequencePurposea
POT2 R55′-CTAGTTCTCATATACATGTATTTGRT-PCR of POT2
POT2 F135′-TGACTACACAAATGTGCAAAGACRT-PCR of POT2
POT1 F15′-GCCTTGACCCTATTCCAATGRT-PCR of POT1
POT1 R15′-ACGTGGTGCTTTTCTCTTTCTRT-PCR of POT1
U1 snRNA F15′-CTTACCTGGCTGGAGTTTGCTATCRT-PCR of U1 snRNA
U1 snRNA R15′-GCGGAGACAGCACTAAGTGCACGRT-PCR of U1 snRNA
R3c5′-CGCAGTTTCGCTGTTCAATA11-kb PCR
F35′-CCCCAACCCCAACCCCAACCCCA11-kb PCR
CBS 4L-6 F15′-TCTTTTACAAAAATTGGCTTTATTGAChIP of CBS 4L-6
CBS 4L-6 R15′-TTTCAGAACACATCACCGATATTTChIP of CBS 4L-7
CBS 3R-2 F15′-AACCAACCTCTTTCGACTTAGGAATChIP of CBS 3R-2
CBS 3R-2 R15′-CAGCAATCAGGAAATAACATACCACChIP of CBS 3R-3
IES M-El F5′-GTGTGGTACAATAGGTTGTCGTAGChIP of IES M-element
IES M-El R5′-TTGAAAGCTAAGTAGCCTTCTTGCChIP of IES M-element
26S rRNA subtel5′-GAACTTCAATCTTTGACTAGCChIP of rDNA subtel
26S rRNA subtel5′-AATTTCTTTGACATTGAGTAAAAGTTATTTATTChIP of rDNA subtel
rDNA internal5′-TGAAATTGCAAGGTAGGTTTCChIP of rDNA int
rDNA internal5′-CATAGTTACTCCCGCCGTTChIP of rDNA int
CBS 4L-6 F5′-GAATTGCATATAAACCAACCTCTTTTTAAATATCGGTGPot1/Pot2 EMSA
CBS 4L-6 R5′-CACCGATATTTAAAAAGAGGTTGGTTTATATGCAATTCPot1/Pot2 EMSA
Tel G-strand5′-TTGGGGTTGGGGTTGGGGTTPot1/Pot2 EMSA
Tel C5′-CCCAACCCCAACCCCAACCCPot1/Pot2 DBD EMSA
Tel G(20)5′-GTTGGGGTTGGGGTTGGGGTPot1/Pot2 DBD EMSA
Tel G(14)5′-GTTGGGGTTGGGGTPot1/Pot2 DBD EMSA
Tet G(12)5′-TGGGGTTGGGGTPot1/Pot2 DBD EMSA
CBS 20 F5′-TATAAAGAGGTTGGTTTATTPot1/Pot2 DBD EMSA
CBS 20 R5′-AATAAACCAACCTCTTTATAPot1/Pot2 DBD EMSA
hTel5′-GGTTAGGGTTAGGGTTAGGGPot1/Pot2 DBD EMSA
Tel Dup G-strand5′-GGCTTAAGC(GGGGTT)7GGGGTPot1/Pot2 DBD EMSA
Tel Dup C-strand5′-ACCCC(AACCCC)4GCTTAAGCCPot1/Pot2 DBD EMSA
  • a subtel, subtelomere; int, internal; EMSA, electrophoretic mobility shift assay; DBD, DNA binding domain.