TABLE 4

Cell wall-related bionumbers and bioestimates of live, haploid, exponential-phase parent cells and budding cells of S. cerevisiae grown in rich medium at 30°C

ParameterValue(s)Reference
Haploid parent yeast cells
    Length and width4.76 and 4.18 μm3
    Surface area60.0 μm2
    Vol43.6 μm3
    Density1.11 g cm−3 (= pg μm−3)88
    Biomass (wet wt)48.4 pg
    Biomass (dry wt)16.5 pg (34% of wet wt)89
    Cellular protein8.7 pg (53% of dry wt)74
    Walls3.5 pg (21% of dry wt)67
    Wall protein0.14 pg (4% of walls)62
    β-Glucan7.7 × 109 Glc62
        β-1,3-Glucan6.1 × 109 Glc62
3.9 × 106 moleculesa62
        β-1,6-Glucan1.6 × 109 Glc
6.6 × 106 moleculesb
    Cellular proteinsc1.0 × 108
    Wall proteins
        No.c1.6 × 106
        Surface density2.7 × 104 μm−2
Haploid budding cells
    Biomass formation0.18 pg min−1; dry wt
    Surface expansion rated0.67 μm2 min−1
    Incorporation rate in bud wallsd
        β-1,3-Glucan6.8 × 107 Glc min−1
        β-1,6-Glucan1.8 × 107 Glc min−1
        Wall proteins1.8 × 104 min−1
  • a Based on an estimated length of 1570 glucose residues (90).

  • b Based on an estimated length of 247 glucose residues (91).

  • c Based on an average protein mass of 52,728 (466 amino acids) (2; BN105224).

  • d Based on a generation time of 90 min and linear growth during that period.