Table 1

Purity of the MLB fraction monitored by immunofluorescencea

Purification stepNo. detected:MLB purity (%)d
MLBsbBacteriac
11,8271899.01
22,0084997.56
33,43210596.9
Avg ± SD97.82 ± 1.08
  • a Observations were made on random fields.

  • b Structures (0.5 to 2 μm) visible by differential interference contrast and presenting DAPI-negative and H36-positive signals.

  • c Structures (∼1 μm) visible by differential interference contrast and presenting DAPI-positive and H36-negative signals.

  • d [no. of MLBs/(no. of MLBs + no. of bacteria)] × 100.