Table 2

Primers used to construct the transformation cassettes

NameaSequenceb (5′→3′)Region
K1-fcacgtcACGCGTcttgcatacaagttctggtgggcgtgTRK1 5′ UTR + TRK1 ORF (from −1694 to 2978)
K2-rtcaggcgtagtcggggacgtcgtaggggtactcgctaaaatcagtaggagtggacgggaaac
K3-fcgacgtccccgactacgcctgatggatgtttcttgttcctatggcgTRK1 3′ UTR (from 2984 to 4261)
K4-rcactagACGCGTctaaccagagccaacatcaccatcctc
K5-fcacgtcGGTACCctcgagtccagtacgagaagatggaagttgagHAK1 5′ UTR (from −1325 to 0)
K6-rggcgtagtcggggacgtcgtaggggtagttgtccatctttttttttatggaagtg
K7-fgacgtccccgactacgccgacactacagcagcccccaaacHAK1 ORF + HAK1 3′ UTR (from 1 to 4199)
K8-rcactagGGTACCgatgggtgtacattctgtacactgggtgttcc
PP1-fcacgtaGCGGCCGCgttgtggttccgtagaggtagPMA1 5′ UTR (from −943 to 0)
PP2-rgtacctTCTAGAtggcgttatggtttgcag
  • a f, forward primer; r, reverse primer.

  • b Uppercase letters designate the restriction sites used for cloning. Italics designate start and stop codons. Coordinates for the DNA fragments are defined from 1 = A of the start codons. Underlining designates the coding sequence for the HA epitope.