Table 2

Primers used in this study

NameSequencea
5′F-Afu5775′-GTGCTCTCTGGTTGGATCAGCCTG-3′
5′R-Afu5785′-GGCTCTTGGGTTGGATTCGAAGG-3′
3′F-Afu5795′-CGTGTTGGCCAGTGGAAAGATTATGTTC-3′
3′R-Afu5805′-CCGCAACGTGGGATAGTGACGC-3′
Afu5PyrG5815′-CCTTCGAATCCAACCCAAGAGCCACCGGTCGCCTCAAACAATGCTCT-3′
Afu3PyrG5825′-GAACATAATCTTTCCACTGGCCAACACGGTCTGAGAGGAGGCACTGATGCG-3′
veAcompFXbaI3945′-AAAAATCTAGATGCTGCTGATCGCCTTGGTC-3′
veAcompRXbaI4785′-AAAAATCTAGAGCGGCTTTTCGCTCACGCTG-3′
pBC-HygroF7695′-ATCCTTGAAGCTGTCCCTGATGGT-3′
pBC-HygroR7705′-TCCGGCTCGTATGTTGTGTGGAAT-3′
veAF398_AscI5′-AAAAGGCGCGCCATGGCGACCAGACCGCC-3′
veAR399_NotI5′-ATATATAAAGCGGCCGCTATGGTAGCGCAGGCAAAATCTTCATGAC-3′
pyrGF-XbaI-14395′-AAAAATCTAGAACCGGTCGCCTCAAACAATGCTCT-3′
pyrGR-XbaI-14395′-AAAAATCTAGAGTCTGAGAGGAGGCACTGATGCG-3′
gpdAF5925′-AAGTACTTTGCTACATCCATACTCC-3′
Afu5′F4285′-AAAGAATTCAAGTTGCAGGAGTAGGATTTGGG-3′
Linker-R6425′-GTCTGAGAGGAGGCACTGATGCG-3′
veA_qRTPCR_F8145′-CCCCACGCTGTCGCAACCTCC-3′
veA_qRTPCR_R8155′-GCCGAAGGTTTCCTCGTGCGATCGC-3′
gliP_qRTPCR_F8405′-AGTTACACCGACTCGCATCCAGCTGC-3′
gliP_qRTPCR_R8415′-CTGGGGCAGACCATGCGTAG-3′
gliZ_qRTPCR_F8385′-ACGACGATGAGGAATCGAACCCG-3′
gliZ_qRTPCR_R8395′-GGTGCTCCAGAAAAGGGAGTCGTTG-3′
veA_gfp_F5895′-GACCGCCTTTAATGCCTCCCG-3′
veA_gfp_R6435′-TGGTAGCGCAGGCAAAATCTTCAT-3′
veA_gfp_fusion_F6445′-ATGAAGATTTTGCCTGCGCTACCAGGAGCTGGTGCAGGCGCTGGAGCC-3′
gliZF_AscI10405′-ATATATATATGGCGCGCCATGGCGACAGCTATGCAGGATGTG-3′
gliZR_Not110415′-ATATATATATAGCGGCCGCCTACAGCGCCAGTAGGTTGCACAATC-3′
18s_qRTPCRF_8495′-TAGTCGGGGGCGTCAGTATTCAGC-3′
18s_qRTPCRR_8505′-GTAAGGTGCCGAGCGGGTCATCAT-3′
brlA_qRTPCRF_11585′-TGCACCAATATCCGCCAATGC-3′
brlA_qRTPCRR_11595′-CGTGTAGGAAGGAGGAGGGGTTACC-3′
FKS1-F5′-GCCTGGTAGTGAAGCTGAGCGT-3′
FKS1-R5′-CGGTGAATGTAGGCATGTTGTCC-3′
qPCR-probe6-FAM-AGCCAGCGGCCCGCAAATG-MGB-3′b
  • a Restriction sites are underlined.

  • b From Integrated DNA Technologies, Coralville, IA. 6-FAM, 6-carboxyfluorescein; MGB, minor groove binder.