Table 1

Analytical models for analysis of the interaction of GXM with chitin

Structural aspect assessedApproach used
Molecular specificity of C. neoformans-chitin interactionPretreatment of the chitin particles with WGA or with soluble GXM; pretreatment of yeasts with anti-GXM
Involvement of noncovalent bondsTreatment of chitin-C. neoformans complexes with urea, NaCl, and EDTA
Requirement of O-acetyl groups of GXMPretreatment of the chitin particles with de-O-acetylated GXM and comparison with results obtained with samples of native GXM; treatment of yeast cells with de-O-acetylating agent, followed by incubation with chitin particles
Requirement of carboxyl groups of GXMPretreatment of the chitin particles with carboxyl-reduced GXM, followed by comparison with results obtained with samples of native GXM
Influence of GXM molecular massTreatment of chitin-C. neoformans complexes with EDTA; pretreatment of chitin particles with fractions containing GXM with different molecular masses
Requirement of chitin amino groupsReplacement of chitin by cellulose in SEM tests
Requirement of N-acetyl groups from chitinPretreatment of yeast cells with chitosan