Table 1

S. cerevisiae strains used in this study

StrainGenotypeaReference
Yeast strains
LH177MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA14
LH185MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA smk1::TRP1C.g/smk1::TRP1C.g.14
LH900MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71::TRP1C.g./spo71::TRP1C.g.This study
LH901MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA SPO71-13 × MYC-TRP1/SPO71-13 × MYC-TRP1This study
LH902MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g.This study
LH903MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA HTB2-mCherry-URA3K.l./HTB2-mCherry-URA3K.l.This study
LH904MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71::TRP1C.g./spo71::TRP1C.g. HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g.This study
LH905MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA SPO71-zz-URA3K.l./SPO71-zz-URA3K.l.This study
LH906MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71(1–1030)-zz-URA3K.l./spo71(1–1030)-zz-URA3K.l.This study
LH907MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71(1–758)-zz-URA3K.l./spo71(1–758)-zz-URA3K.l.This study
LH908MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71(1–1030)-zz-URA3K.l./spo71(1–1030)-zz-URA3K.l. HTB2-mCherry-URA3K.l./HTB2-mCherry-URA3K.l.This study
LH909MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71(1–758)-zz-URA3K.l./spo71(1–758)-zz-URA3K.l. HTB2-mCherry-URA3K.l./HTB2-mCherry-URA3K.l.This study
LH790MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA DON1-GFP-HIS3MX6/DON1-GFP-HIS3MX6This study
LH910MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA DON1-GFP-HIS3MX6/DON1-GFP-HIS3MX6 spo71::TRP1C.g./spo71::TRP1C.g.This study
LH911MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g. SPR28-3XGFP-KANMX6/SPR28-3XGFP-KANMX6This study
LH912MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g. SPR28-3XGFP-KANMX6/SPR28-3XGFP-KANMX6 spo71::TRP1C.g./spo71::TRP1C.g.This study
LH913MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo1::HIS3C.g./spo1::HIS3C.g.This study
LH914MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo1::HIS3 C.g./spo1::HIS3 C.g. spo71::TRP1C.g./spo71::TRP1C.g.This study
LH915MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo1::HIS3C.g./spo1::HIS3C.g. HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g.This study
LH916MATa/MATα ho::hisG/ho::hisG lys2/lys2 ura3/ura3 leu2/leu2 his3/his3 trp1ΔFA/trp1ΔFA spo71::TRP1C.g./spo71::TRP1C.g. spo1::HIS3C.g./spo1::HIS3C.g. HTB2-mCherry-TRP1C.g./HTB2-mCherry-TRP1C.g.This study
Yeast strains with episomal plasmids
LH917LH902 plus pRS426-G20This study
LH918LH903 plus pRS424-G20This study
LH919LH904 plus pRS426-G20This study
LH920LH908 plus pRS424-G20This study
LH921LH909 plus pRS424-G20This study
LH922LH790 plus pRS426-M20This study
LH923LH910 plus pRS426-M20This study
LH924LH904 plus pRS426-G71(1–1245)This study
LH925LH904 plus pRS426-G71(1–1037)This study
LH926LH904 plus pRS426-G71(1–758)This study
LH927LH904 plus pRS426-G71(753–1037)This study
LH928LH904 plus pRS426-G71(963–1245)This study
LH929LH904 plus pRS426-G71(753–1245)This study
LH931LH915 plus pRS426-G20This study
LH932LH916 plus pRS426-G20This study
  • a C.g., Candida glabrata; K.l., Kluyveromyces lactis.