Table 1

LC-MS/MS analysis of polar tube protein tryptic peptides of N. bombycis

ProteinGenBank accession no.pI/molecular mass (kDa)% CoverageLC-MS/MS peptide mass hitPeptide sequenceaDifference (MH+) (Da)
m/zOverlap/totalChargeMolecular mass (MH+) (Da)
PTP2HQ8814989.69/31.456.121,172.722/3211,841.09740AKEAYVFNAIGEVLSTK1.29640
1,032.415/2021,280.44880AVCNIQYINGK−2.73520
2,170.023/2821,641.84630EAYVFNAIGEVLSTK0.56030
211.517/2621,611.73530IIPANNNNPAECQR−1.04170
777.419/2421,552.86890IM*LAANIQHHFIK1.20290
1,175.017/2421,536.86950IMLAANIQHHFIK1.29550
809.018/2821,739.90820KIIPANNNNPAECQR1.25520
1,249.526/5231,681.04180KIM*LAANIQHHFIK−0.47120
1,214.431/7232,221.40690LLNELKNEPEYTVTGEENK2.29490
1,846.536/9232,809.12070LLNELKNEPEYTVTGEENKVNVFK0.55470
646.918/2421,510.54160NEPEYTVTGEENK0.48860
975.222/3422,098.25540NEPEYTVTGEENKVNVFK0.80340
1,251.424/4222,467.67000NYDLFVNILSNTTVSEPGPEEK0.11100
674.918/4422,595.84290NYDLFVNILSNTTVSEPGPEEKK−0.61110
397.618/3021,746.89110STPQTAEGTPLINECK0.56610
733.315/2421,469.69190AQQQM*LPAVVDPR0.22090
1,252.418/2421,453.69250AQQQMLPAVVDPR0.36150
1,527.616/2221,408.62170KAVCNIQYINGK0.48770
824.915/1821,059.15860QAQAIETAAR0.23160
PTP3JF7395546.73/150.34.56897.417/3011,800.94380TFIDEEVSNVGEAYVK0.01280
771.716/2821,712.83820YPTAVADEEFDSLVR−0.36580
947.219/3422,015.23310ALADSLGMTEEDFIQFAR0.58210
297.115/2021,272.39010AVAELQDEIQR−0.66390
1,221.822/4222,099.24820SDQVIAGPNGGTSALSQATAPR−0.27280
1,059.713/161902.97550VAAENATAR0.15650
535.713/2421,467.71600VGSLITDFNTMLR−1.03300
  • a *, methionine oxidation.