Table 2.

Known bradyzoite and tachyzoite developmentally regulated genes

Probe setGene IDGene nameProductAvg expressionaFold changeb
2dT4dB8dB21dB4dB vs 2dT8dB vs 2dT21dB vs 2dT21dB vs 4dB
Upregulated in bradyzoites versus tachyzoites
    55.m00009_atTGME49_059020BAG1Heat shock protein, bradyzoite antigen6.29.99.410.0291834
    59.m03411_atTGME49_068860ENO1Enolase 15.28.68.59.31816362.0
    583.m09133_atTGME49_114250BRP1Bradyzoite rhoptry protein5.08.27.68.8137.223
    23.m00149_s_atTGME49_007130SAG2YSurface antigen (SRS49A)4.86.26.07.22.32.05.42.3
    641.m01562_atTGME49_120190SRS9Surface antigen (SRS16B)4.15.55.65.62.02.02.1
    80.m00010_atTGME49_091040LDH2Lactate dehydrogenase 24.97.87.79.29.18.5343.8
Downregulated in bradyzoites versus tachyzoites
    59.m03410_atTGME49_068850ENO2Enolase 27.34.65.34.20.150.210.13
    59.m00008_atTGME49_071050SAG2ASurface antigen (SRS34A, P22)8.46.97.26.00.240.12
    44.m00010_atTGME49_033480SRS2Surface antigen (SRS29C, P35)7.65.05.34.20.140.160.10
    44.m00009_atTGME49_033460SAG1Surface antigen (SRS29B, P30)9.35.97.14.60.050.120.02
    44.m00006_atTGME49_032350LDH1Lactate dehydrogenase 16.05.45.55.10.55
  • a 4dB, 4-dpi bradyzoites; 8dB, 8-dpi bradyzoites; 21dB, 21-dpi bradyzoites; 2dB, 2-dpi tachyzoites. Average normalized glog-transformed expression values.

  • b Fold change calculated from glog mean expression values back-transformed to the original scale (see Materials and Methods) and shown only where values are significantly different (P < 0.05). —, fold change not significant.