Table 1.

Summary of Neurospora serine-threonine protein kinase groups, families, and phenotypes

GroupaFamilybNCUno.cNeurospora genedS. cerevisiae homologePhenotype summary
InviableGrowth of basal hyphaefAsexual developmentgSexual developmenthChemical sensitivityi
AGCAKT03200stk-10SCH9RAHSC
AGCAKT07280stk-50YPK1/YPK2Xj
AGCNDR03242stk-21
AGCNDR07296cot-1 (95)CBK1R
AGCNDR07378stk-12RIM15AH
AGCNDR09071dbf-2 (20)DBF2/DBF20RC, AHPP, P, ANSk
AGCPDK1/PKA03571stk-23PKH1/PKH2RT
AGCPKA00682pkac-2 (5)AH
AGCPKA06240pkac-1 (5)TPK1/TPK2/TPK3RC, AHPP, PNS
AGCPKC06544pkc (23)PKC1X
AGCRSK01797nrc-2 (49)KIN82/FPK1RC, AHP, ANS
AGCRSK/AKT03197stk-11YPK3
AGCYANK07062stk-49
CAMKCAMK102283camk-2
CAMKCAMK109123camk-1 (94)CMK1/CMK2RC, AHPP, P, ANS
CAMKCAMK109212camk-4RCK1/RCK2B
CAMKCAMKL00914stk-16FRK1/KIN4RPP, P, AB, SC, FL
CAMKCAMKL02245stk-19YPL150WP, AN/S
CAMKCAMKL04566prk-10SNF1SC, T
CAMKCAMKL04747stk-31KIN1/KIN2RC, AHPP, P, ANS
CAMKCAMKL06249stk-40PSK1/PSK2
CAMKCAMKL08346mus-58 (88)CHK1AHSC
CAMKCAMKL09064stk-53
CAMKCAMKL/CAMK unique04143stk-26PRR1
CAMKRAD5302751mus-59 (88)
CAMKRAD5302814prd-4 (73)DUN1
CAMKCAMK1/RAD53/CAMKL06486stk-43
CK1CK100685ck-1a (27)HRR25X
CK1CK104005ck-1b (93)YCK1/YCK2/YCK3X
CMGCCDK01435stk-1X
CMGCCDK03659prk-3KIN28T
CMGCCDK04426div-4CAK1RPP, P, AM, NS
CMGCCDK06685stk-47CTK1RPP, P, AC
CMGCCDK07172stk-8SSN3RC, AHPP, P, ANS
CMGCCDK07580mdk-1PHO85X
CMGCCDK07880prk-6
CMGCCK203124cka (60)CKA1/CKA2AHSC
CMGCCLK00230prk-4KNS1X
CMGCDYRK06638stk-46RPP, P, AM
CMGCDYRK07872prk-2YAK1RC, AHPP, P, ANS
CMGCDYRK10853stk-57
CMGCGSK04185gsk-3 (20)RIM11/MRK1B
CMGCMAPK02393mak-2 (53)FUS3/KSS1RC, AHPP, P, ANS
CMGCMAPK07024os-2 (92)HOG1C,PP, P, ANS
CMGCMAPK09842mak-1 (56, 68)SLT2RC, AHPP, P, AN/S
CMGCRCK01498ime-2 (38)IME2RAHF
CMGCSRPK09202mdk-2SKY1RC, AHPP, P, ANS
CMGCCLK/SRPK05655stk-35RAHPP, P, ANS
CMGCCLK/SRPK05658stk-36RC, AHPP, P, ANS
CMGCCLK/SRPK/DYRK09189stk-54C
CMGCCLK/SRPK10004stk-56
OtherAUR00108stk-13IPL1X
OtherCAMKK03523stk-22TOS3/SAK1RAHC, F
OtherCAMKK06177camk-3
OtherCDC711410cdc7CDC7NAlNANANANA
OtherHAL01940ptk-2 (52)PTK1/PTK2RF
OtherHAL04335stk-30NPR1/PRR2SC
OtherHAL06179stk-5SAT4SC
OtherIRE02202stk-14IRE1
OtherNAK06202stk-38ARK1/PRK1RC, AHPP, PC, B, M
OtherNAK07399stk-9YPL236CPC, AHPP, P, AF, T, M
OtherNEK03187nim-1 (75)KIN3AB
OtherPEK01187cpc-3 (78)GCN2R
OtherPLK09258cdc5CDC5X
OtherRAN04990stk-17/fi (59)SKS1/VHS1RC, AHPP, P, AT
OtherRAN06230stk-39
OtherSCYI04755stk-32SCY1
OtherULK00188apg-1ATG1RAHPP, P, AF, S, SC, T, M
OtherVPS1506626stk-45VPS15
OtherWEE04326stk-29SWE1B
OtherIKS08177stk-51IKS1
STESTE1101335cdc15PP, P, A
STESTE1102234mik-1 (68)BCK1RC, AHPP, P, ANS
STESTE1103071os-4 (24, 42)SSK2/SSK22C,PP, P, ANS
STESTE1106182nrc-1 (49)STE11RC, AHPP, P, ANS
STESTE2000406vel (this study)CLA4/SKM1RC, AHPP, ANS
STESTE2000772mst-1 (20)A
STESTE2003894stk-4STE20RT, M
STESTE2004096prk-9RC, AHPP, P, ANS
STESTE2011235pod-6 (79)NANANANANA
STESTE700587os-5 (24)PBS2C,PP, P, ANS
STESTE704612mek-2 (56)STE7RC, AHPP, P, ANS
STESTE706419mek-1 (68)MKK1/MKK2NANANANANA
Unclassified02885stk-20
Unclassified05638stk-34
Unclassified06006stk-37
Unclassified06421stk-41
Unclassified06422stk-42
Unclassified06583stk-44
CMGCCDK09778cdc28CDC28
OtherBUD3204595prk-11BUD32
OtherSCY104279stk-28CEX1
AtypicalABC103823stk-25ABC1
AtypicalABC104259stk-27YLR253W
AtypicalABC105600stk-33YPL109C
AtypicalBRD09595stk-55
AtypicalPDHK03796stk-24YGL059W
AtypicalPDHK06760stk-48
AtypicalPDHK11744stk-58PKP1
AtypicalPIKK00274mus-21 (87)TEL1
AtypicalPIKK01379stk-18TRA1
AtypicalPIKK05608div-18TOR1/TOR2
AtypicalPIKK11188mus-9 (87)MEC1
AtypicalRIO07722rgb-40RIO2
AtypicalRIO08767stk-52RIO1
AtypicalTAF102556hat-2TAF1
TKLLRRK05808tkl-1
  • a Group abbreviations: AGC, PKA (protein kinase A/cyclic AMP-dependent protein kinase), PKG (protein kinase G/cGMP-dependent protein kinase), PKC (protein Kinase C); CK1 (casein kinase 1 or cell kinase 1); CAMK (Ca2+/calmodulin-dependent protein kinase); CMGC, cyclin-dependent, mitogen-activated, glycogen synthase and cyclin-dependent protein kinase-like kinases; STE, sterile; TKL, tyrosine kinase-like kinase.

  • b Family abbreviations: AKT, oncogene protein of v-akt; NDR, nuclear Dbf2 related; PDK1, phosphoinoside-dependent protein kinase; PKA, protein kinase A or cyclic AMP-dependent protein kinase; PKC, protein kinase C; RSK, ribosomal s6 kinase; YANK, yet another novel kinase; CAMK, Ca2+/calmodulin-dependent protein kinase; CAMKL, Ca2+/calmodulin-dependent protein kinase-like kinase; RAD, radiation sensitive; CK, casein kinase or cell kinase; CDK, cyclin-dependent protein kinase; CLK, cyclin-dependent protein kinase-like kinase; DYRK, dual-specificity tyrosine phosphorylation-regulated kinase; GSK, glycogen synthase kinase; MAPK, mitogen-activated protein kinase; RCK, related to murine RCK (ros cross-hybridizing kinase); SRPK, serine-rich protein kinase; AUR, Aurora; CAMKK, Ca2+/calmodulin-dependent protein kinase kinase; CDC, cell division cycle; HAL, halotolerance; IRE, inositol-requiring protein; NAK, NF-κB-activating kinase; NEK, NIMA-related kinase; PEK, pancreatic alpha-subunit of eukaryotic initiation factor kinase; PLK, polo kinase; RAN, Ran GTPase kinase; SCY1, related to S. cerevisiae Scy1 kinase; ULK, Unc-51-like kinase; VPS, vacuolar protein sorting; WEE, small; IKS, Ira1 kinase suppressor; STE, sterile; ABC1, related to S. cerevisiae Abc1 kinase; BRD, bromodomain; PDHK, pyruvate dehydrogenase kinase; PIKK, phosphatidyl inositol 3′-kinase-related kinase; RIO, right open reading frame; TAF1, TATA-binding protein-associated factor 1; LRRK, leucine-rich repeat kinase.

  • c Based on version 5 annotation of the Broad Institute's Neurospora crassa database (www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html).

  • d References for previously studied genes are in parentheses. Kinase names are consistent with the Neurospora e-Compendium Project at Leeds University (http://bmbpcu36.leeds.ac.uk/∼gen6ar/newgenelist/genes/gene_list.htm). stk-18 through stk-58 were named during this study, in accordance with the e-Compendium system.

  • e Yeast orthologs were obtained from the literature or assigned during this study based upon analyses of phylogenetic trees available at www.phylomedb.org (34, 35).

  • f R, reduced growth.

  • g Asexual phenotypes are represented by phenotypes in aerial hyphae (AH) or conidial development (C).

  • h The sexual phenotypes are represented by their occurrence during protoperithecial (PP), perithecial (P), or ascospore (A) development.

  • i Chemical phenotypes are summarized based on sensitivity or resistance to cytochalasin A (C), benomyl (B), FK-506 (F), sorbitol (S), sodium chloride (SC), fludioxonil (FL), tert-butyl hydroperoxide (T), and menadione (M).

  • j —, phenotypic assay could not be performed due to inviability of knockout mutant.

  • k NS, mutant was not analyzed by chemical screening due to poor growth.

  • l NA, mutant was not analyzed for phenotypes due to simultaneous mutation of adjacent gene.