Table 1.

Morphological characteristics, maximum dimension of surface subunits, and cell wall viscoelastic moduli of wild-type and Galf biosynthesis enzyme gene deletion strains

StrainHyphal widtha (μm) (mean ± SE)Wall thicknessb (nm) (mean ± SE)Subunit maximum dimension (nm) (mean ± SD)Viscoelastic moduli (MPa) (mean ± SD)c of:Adhesion (nN) (mean ± SD)
Fixed hyphal wallLive hyphal wall
Wild-type (AAE1)2.4 ± 0.054 ± 235 ± 5211 ± 1582.3 ± 12.95.7 ± 1.6
ugeAΔ3.6 ± 0.1104 ± 1063 ± 1099 ± 4824.6 ± 13.7ND
ugeBΔ2.5 ± 0.095 ± 1139 ± 874 ± 2222.5 ± 8.6ND
ugeAΔ ugeBΔ3.5 ± 0.1217 ± 62NDd38 ± 219.8 ± 5.1ND
ugmAΔ3.1 ± 0.1204 ± 10108 ± 3514 ± 23.1 ± 0.48.1 ± 0.3
ugeAΔ ugmAΔ3.2 ± 0.4162 ± 897 ± 230.05 ± 0.020.03 ± 0.0117.3 ± 3.9
  • a There was no significant difference in the hyphal width for fixed or live hyphae measured by either confocal microscopy or atomic force microscopy (AFM).

  • b Measured from TEM hyphal cross sections. See Materials and Methods.

  • c Standard deviations (SDs) were calculated from the standard errors propagated through equations 1 and 2. See Materials and Methods.

  • d ND, not determined. See Results.