Table 1.

Strains used in this study

StrainGenotypeReference
BY4741 (YSC1048)MATa leu2Δ ura3Δ his3Δ met15ΔYeast deletion collection (Open Biosystems)
BY4742 (YSC1049)MATα leu2Δ ura3Δ his3Δ lys2ΔYeast deletion collection (Open Biosystems)
VHY70MATa leu2Δ ura3Δ his3Δ24
VHY60MATa leu2Δ ura3Δ his3Δ met15Δ hph1Δ::KanMX4 hph2Δ::KanMX424
hph1Δ (no. 1621)Isogenic to BY4741Yeast deletion collection (Open Biosystems)
hph2Δ (no. 380)Isogenic to BY4741Yeast deletion collection (Open Biosystems)
pkr1Δ (no. 6564)Isogenic to BY4741Yeast deletion collection (Open Biosystems)
sec71Δ (no. 3311)Isogenic to BY4741Yeast deletion collection (Open Biosystems)
hph1Δ hph2Δ sec71ΔMATa leu2Δ ura3Δ his3Δ hph1Δ::KanMX4 hph2Δ::KanMX4 sec71Δ::KanMX4This study
hph1Δ hph2Δ pkr1ΔMATa leu2Δ ura3Δ his3Δ hph1Δ::KanMX4 hph2Δ::KanMX4 pkr1Δ::KanMX4This study
vma21Δ (no. 4735)Isogenic to BY4741Yeast deletion collection (Open Biosystems)
BY4743-SEC71-TAPMATaleu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC71::TAP::HIS3MX6/SEC71This study
BY4743-SEC72-TAPMATaleu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC72::TAP::HIS3MX6/SEC72This study
BY4743-SEC61-TAPMATaleu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lys2Δ/LYS2 SEC61::TAP::HIS3MX6/SEC61This study
BY4743-SEC62-TAPMATaleu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lysΔ2/LYS2 SEC62::TAP::HIS3MX6/SEC62This study
BY4743-SEC63-TAPMATaleu2Δ/leu2Δ ura3Δ/ura3Δ met15Δ/MET15 lysΔ2/LYS2 SEC63::TAP::HIS3MX6/SEC63This study