Table 3.

Oligonucleotides used in this study

NameSequence (5′–3′)
PilAΔ-Ribo FGCA GAA TAT CGG CTG GTC TC CGC TCT AGA ACT AGT GGA TCC
PilAΔ-Ribo RGCT CAT TCA GTG AGT GCT CG CCT CGA GGT CGA CGG TAT CG
PilBΔ-PyrG FCAC CTG TCT AGC CTC AGC AA GTC GCC TCA AAC AAT GCT CTT C
PilBΔ-PyrG RCAC AAA GCA CTA ATC ACC CCT T CTG AGA GGA GGC ACT GAT GC
SurGΔ-Pyro FGTC TAC TCG TCT CTC ATC AGA CGC TCT AGA ACT AGT GGA TCC
SurGΔ-Pyro RGTG TAT AGC CGA CAG CAG CA CCT CGA GGT CGA CGG TAT CG
P1 PilAΔCGA TCC TAG CTC TCA GGA TC
P3 PilAΔGGA TCC ACT AGT TCT AGA GCG GAG ACC AGC CGA TAT TCT GC
P4 PilAΔCGA TAC CGT CGA CCT CGA GG CGA GCA CTC ACT GAA TGA GC
P6 PilAΔACC AAC CTA GTC GAC GTG AC
P1 PilBΔCTC AGC TGA GAG ACT GTC AG
P3 PilBΔGAA GAG CAT TGT TTG AGG CGA C TTG CTG AGG CTA GAC AGG TG
P4 PilBΔGCA TCA GTG CCT CCT CTC AG AAG GGG TGA TTA GTG CTT TGT G
P6 PilBΔTCG GAG TCA ATG TAG TAC AGC
P1 SurGΔGCT CAC ATC CAC AAT GTC TAG
P3 SurGΔGGA TCC ACT AGT TCT AGA GCG TCT GAT GAG AGA CGA GTA GAC
P4 SurGΔCGA TAC CGT CGA CCT CGA GG TGC TGC TGT CGG CTA TAC AC
P6 SurGΔGAC TGC CAC ACC TCA CCT C
P2 PilAΔGCT GAA CCA GAA GAG GCT GC
P5 PilAΔGCA TCC ATG ATG TCA GCA TAC
P2 PilBΔTCA TCA CCA GGC AAG ATC ATC
P5 PilBΔCAT CGT TCC CAT GCT CAG AC
P2 SurGΔTCT TGT GCT GAG GGA ACT AAG
P5 SurGΔCGT CCT CAT CCG TGT CTG C
PilA FCGG GAG CCT GTC CCT GTC GGA GCT GGT GCA GGC GCT G
PilA RGCT CAT TCA GTG AGT GCT CG CTG AGA GGA GGC ACT GAT GC
PilA P1GCA TCG TAC ATA CTC TAT GCG
PilA P2CAC CAC TCT CCT CGA CCA AG
PilA P3CAG CGC CTG CAC CAG CTC C GAC AGG GAC AGG CTC CCG
PilA P4GCA TCA GTG CCT CCT CTC AG CGA GCA CTC ACT GAA TGA GC
PilA P5GCA TCC ATG ATG TCA GCA TAC
PilA P6ACC AAC CTA GTC GAC GTG AC
PilB FAGC GAG TTG CAG TGC CCA TT GGA GCT GGT GCA GGC GCT G
PilB RCAC AAA GCA CTA ATC ACC CCT T CTG AGA GGA GGC ACT GAT GC
PilB P1GCA GAA GAA GGA GCT CTG TC
PilB P2CGT GCT GAT GGC AGA AAT GG
PilB P3CAG CGC CTG CAC CAG CTC C AAT GGG CAC TGC AAC TCG CT
PilB P4GCA TCA GTG CCT CCT CTC AG AAG GGG TGA TTA GTG CTT TGT G
PilB P5CAT CGT TCC CAT GCT CAG AC
PilB P6TCG GAG TCA ATG TAG TAC AGC
SurG FGCA ACA AGG AAA TCG CTC CCG GAG CTG GTGCAG GCG CTG GAG
SurG RGTG TAT AGC CGA CAG CAG CAC TGA GAG GAG GCA CTG ATG CGT G
SurG P1CTT CAT CGT TCA AGC TTC AGG
SurG P2TCT ACT CGT CTC TCA TCA GAG
SurG P3C TCC AGC GCC TGC ACC AGC TCC GGG AGC GAT TTC CTT GTT GC
SurG P4CAC GCA TCA GTG CCT CCT CTC AG TGC TGC TGT CGG CTA TAC AC
SurG P5TGT CGA GTT TCT GCC TCT CC
SurG P6GAC TGC CAC ACC TCA CCT C