Table 1.

A. nidulans, E. coli, and S. cerevisiae strains used in this study

StrainGenotypeSource
AJC1.5biA1 apsB6J. Clutterbuck (1969)
AJC1.7biA1 apsB10J. Clutterbuck (1969)
FGSC89biA1 argB2FGSC
GJA28biA1 ΔhexA::argB (FGSC89 transformed with ΔhexA::argB deletion cassette)G. Jedd, Singapore
GR5pyrG89 wA3 pyroA438
MH11269biA1 niiA4 pyroA4 pexC::bar12
SDV38alcA(p)::GFP::hexA680 wA3 pyroA4 (GR5 transformed with pDV15)This work
SDV42alcA(p)::YFPN::apsB3.2 alcA(p)::YFPC::hexA680 wA3 pyroA4 (GR5 transformed with pDV17 and pDV22b)This work
SDV43alcA(p)::YFPN::hexA1.0 alcA(p)::YFPC::apsB3.2 wA3 pyroA4 (GR5 transformed with pDV19a and pDV23a)This work
SDV49-4alcA(p)::mRFP1::apsB1.5 alcA(p)::GFP::hexA680 pyroA4 ΔnkuA::argB (TN02A3 transformed with pDV15 and pDM8a)This work
SDV70byA1 pyroA4 riboB2 areA102 gpd(p)::GFP::acuE alcA(p)::mRFP1::apsB (TALX207-10 transformed with pDV42a)This work
SDV73alcA(p)::GFP::apsB1.5 alcA(p)::mRFP1::hexA680 pyroA4 ΔnkuA::argB [TN02A3 transformed with pDV39 and pMCB17apx(-apsB)]This work
SDV77alcA(p)::GFP::apsB-PTS2.1 pyroA4 ΔnkuA::argB (TN02A3 transformed with pDV43)This work
SDV78calcA(p)::mRFP1::hexA680 gpd(p)::GFP::acuE yA1 riboB2 areA102 (TALX207-10 transformed with pDV39 and pTN1)This work
SDV79ΔhexA alcA(p)::GFP::apsB_PTS2mut (GJA28 crossed with SDV77a)This work
SDV80apsB6 alcA(p)::GFP::apsB_PTS2mut (AJC1.5 crossed with SDV77)This work
SDV88apsB6 alcA(p)::GFP::apsB1.5 (AJC1.5 crossed with SEa3)This work
SDV95ΔhexA ΔapsB alcA(p)::GFP::apsB_PTS2mut (SDV82 crossed with SRS25)This work
SDV98apsB10 alcA(p)::GFP::tubA (AJC1.7 crossed with SJW02)This work
SDV103apsB10 alcA(p)::GFP::tubA alcA(p)::GFP::apsB_PTS2mut (SDV98 transformed with pDV43a)This work
SEa3alcA(p)::GFP::apsB wA3 pyroA435
SJW02alcA(p)::GFP::tubA ΔargB::trpCΔB wA3 pyroA435
SNZ11alcA(p)::YFPN::apsB3.2 alcA(p)::YFPC::γtubulin1.8 wA3 pyroA4 (GR5 transformed with pDV22 and pDV50)This work
SNZ16alcA(p)::GFP::γtubulin1.8 pyroA4 (TN02A3 transformed with pNZ17)This work
SNZ22alcA(p)::GFP::γtubulin1.8 gpd(p)::DsRed::stuA(NLS) (SNZ16 transformed with pJH19 and pTN1)This work
SNZ34apsB10 alcA(p)::GFP::apsB_PTS2mut_SRL (AJC1.7 transformed with pNZ16)This work
SNZ37alcA(p)::GFP::γtubulin apsB::3×HA (SNZ16 transformed with pNZS23 and pTN1)This work
SNZ59apsB(p)::GFP::apsB pyroA4 (TN02A3 transformed with pNZ-SI37)This work
SNZ61γtubulin(p)::GFP::γtubulin pyroA4 (TN02A3 transformed with pNZ-SI36)This work
SNZ94pabaA1 biA1 alcA(p)::GFP::apsB_PTS2mut_SRL gpd(p)::GFP:stuA(NLS) ΔapsB::argB trpC801 (SRS24 transformed with pNZ16)This work
SNZ-SH80alpB(p)::alpB::GFP pyroA4 ΔnkuA::argB (SO451 transformed with alpB::GFP::pyrG::RB-alpB fusion PCR)This work
SNZ-SI 42alcA(p)::3×HA::apsB pyroA4 (TN02A3 transformed with pSI-N4)This work
SO451pyrG89 wA3 pyroA4 ΔnkuA::argBFGSC
SRS24gpd(p)::GFP::stuA(NLS) pabaA1 ΔapsB::argB trpC80131
TALX207-10yA1 pyroA4 areA102 transformed with gpd(p)::GFP::acuE and riboB+ plasmidM. Hynes and A. Andrianopoulos, Melbourne, Australia
TNO2A3pyrG89 pyroA4 ΔnkuA::argBS. Osmani
PJ69-4AMATa trp1-901 leu2-3 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE-LYS::GAL1-HIS3 met2::GAL7-lacZ2
AH109MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-lacZ GAL2-ADE-LYS::GAL1-HIS3 met2::GAL7-lacZ13
Y187MATα ura3-52 his3-200 ade2-101 trp1-901 leu2-3,112 gal4Δ met-gal80Δ URA3::GAL1UAS-GAL1TATA-lacZP. Uetz, Karlsruhe, Germany
  • a FGSC, Fungal Genetics Stock Center.