Table 1.

A. nidulans, E. coli, and S. cerevisiae strains used in this study

StrainGenotypeSource
AJC1.5 biA1 apsB6 J. Clutterbuck (1969)
AJC1.7 biA1 apsB10 J. Clutterbuck (1969)
FGSC89 biA1 argB2 FGSC
GJA28 biA1 ΔhexA::argB (FGSC89 transformed with ΔhexA::argB deletion cassette)G. Jedd, Singapore
GR5 pyrG89 wA3 pyroA4 38
MH11269 biA1 niiA4 pyroA4 pexC::bar 12
SDV38 alcA(p)::GFP::hexA680wA3 pyroA4 (GR5 transformed with pDV15)This work
SDV42 alcA(p)::YFPN::apsB3.2alcA(p)::YFPC::hexA680wA3pyroA4 (GR5 transformed with pDV17 and pDV22b)This work
SDV43 alcA(p)::YFPN::hexA1.0alcA(p)::YFPC::apsB3.2wA3 pyroA4 (GR5 transformed with pDV19a and pDV23a)This work
SDV49-4 alcA(p)::mRFP1::apsB1.5alcA(p)::GFP::hexA680pyroA4 ΔnkuA::argB (TN02A3 transformed with pDV15 and pDM8a)This work
SDV70b yA1 pyroA4 riboB2 areA102 gpd(p)::GFP::acuE alcA(p)::mRFP1::apsB (TALX207-10 transformed with pDV42a)This work
SDV73 alcA(p)::GFP::apsB1.5alcA(p)::mRFP1::hexA680pyroA4 ΔnkuA::argB [TN02A3 transformed with pDV39 and pMCB17apx(-apsB)]This work
SDV77 alcA(p)::GFP::apsB-PTS2.1 pyroA4 ΔnkuA::argB (TN02A3 transformed with pDV43)This work
SDV78c alcA(p)::mRFP1::hexA680gpd(p)::GFP::acuE yA1 riboB2 areA102 (TALX207-10 transformed with pDV39 and pTN1)This work
SDV79ΔhexA alcA(p)::GFP::apsB_PTS2mut (GJA28 crossed with SDV77a)This work
SDV80 apsB6 alcA(p)::GFP::apsB_PTS2mut (AJC1.5 crossed with SDV77)This work
SDV88 apsB6 alcA(p)::GFP::apsB1.5 (AJC1.5 crossed with SEa3)This work
SDV95ΔhexA ΔapsB alcA(p)::GFP::apsB_PTS2mut (SDV82 crossed with SRS25)This work
SDV98 apsB10 alcA(p)::GFP::tubA (AJC1.7 crossed with SJW02)This work
SDV103 apsB10 alcA(p)::GFP::tubA alcA(p)::GFP::apsB_PTS2mut (SDV98 transformed with pDV43a)This work
SEa3 alcA(p)::GFP::apsB wA3 pyroA4 35
SJW02 alcA(p)::GFP::tubA ΔargB::trpCΔB wA3 pyroA4 35
SNZ11 alcA(p)::YFPN::apsB3.2alcA(p)::YFPC::γtubulin1.8wA3 pyroA4 (GR5 transformed with pDV22 and pDV50)This work
SNZ16 alcA(p)::GFP::γtubulin1.8pyroA4 (TN02A3 transformed with pNZ17)This work
SNZ22 alcA(p)::GFP::γtubulin1.8gpd(p)::DsRed::stuA(NLS) (SNZ16 transformed with pJH19 and pTN1)This work
SNZ34 apsB10 alcA(p)::GFP::apsB_PTS2mut_SRL (AJC1.7 transformed with pNZ16)This work
SNZ37 alcA(p)::GFP::γtubulin apsB::3×HA (SNZ16 transformed with pNZS23 and pTN1)This work
SNZ59 apsB(p)::GFP::apsB pyroA4 (TN02A3 transformed with pNZ-SI37)This work
SNZ61γtubulin(p)::GFP::γtubulin pyroA4 (TN02A3 transformed with pNZ-SI36)This work
SNZ94 pabaA1 biA1 alcA(p)::GFP::apsB_PTS2mut_SRL gpd(p)::GFP:stuA(NLS) ΔapsB::argB trpC801 (SRS24 transformed with pNZ16)This work
SNZ-SH80 alpB(p)::alpB::GFP pyroA4 ΔnkuA::argB (SO451 transformed with alpB::GFP::pyrG::RB-alpB fusion PCR)This work
SNZ-SI 42 alcA(p)::3×HA::apsB pyroA4 (TN02A3 transformed with pSI-N4)This work
SO451 pyrG89 wA3 pyroA4 ΔnkuA::argBFGSC
SRS24 gpd(p)::GFP::stuA(NLS) pabaA1 ΔapsB::argB trpC801 31
TALX207-10 yA1 pyroA4 areA102 transformed with gpd(p)::GFP::acuE and riboB+ plasmidM. Hynes and A. Andrianopoulos, Melbourne, Australia
TNO2A3 pyrG89 pyroA4 ΔnkuA::argBS. Osmani
PJ69-4A MAT a trp1-901 leu2-3 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE-LYS::GAL1-HIS3 met2::GAL7-lacZ 2
AH109 MAT a trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-lacZ GAL2-ADE-LYS::GAL1-HIS3 met2::GAL7-lacZ 13
Y187 MATα ura3-52 his3-200 ade2-101 trp1-901 leu2-3,112 gal4Δ met-gal80Δ URA3::GAL1UAS-GAL1TATA-lacZP. Uetz, Karlsruhe, Germany
  • a FGSC, Fungal Genetics Stock Center.