Plasmids used in this study
| Plasmid | Construction | Source |
|---|---|---|
| pCR2.1-TOPO | Cloning vector | Invitrogen |
| pDM8a | GFP replaced with mRFP1 in pMCB17apx-apsB | 35 |
| pDV7 | alcA(p)::YFPN::apsB1.5 pyr4 GFP of pMCB17apx-apsB replaced with YFPN | This work |
| pDV8 | alcA(p)::YFPC::apsB1.5 pyr4 GFP of pMCB17apx-apsB replaced with YFPC | This work |
| pDV15 | alcA(p)::GFP::hexA680 pyr4 pMCB17apx with full-length hexA | This work |
| pDV17 | alcA(p)::YFPC::hexA680 pyr4 apsB of pDV8 replaced with hexA680 | This work |
| pDV19 | alcA(p)::YFPN::hexA1.0 pyr4 apsB of pDV7 replaced with full-length hexA1.0 | This work |
| pDV21a | pMCB17-apx containing full-length apsB of 3.2 kb between AscI and PacI restriction sites; alcA(p)::GFP::apsB3.2 pyr4 | This work; 35 |
| pDV22b | alcA(p)::YFPN::apsB3.2 pyr4 apsB of pDV7 replaced with full-length apsB3.2 | This work |
| pDV23 | alcA(p)::YFPC::apsB3.2 pyr4 apsB of pDV8 replaced with full-length apsB3.2 | This work |
| pDV39 | alcA(p)::mRFP1::hexA680 pyr4 apsB of pDM8a changed with hexA680 | 35 |
| pDV42a | alcA(p)::mRFP::apsB3.2 pyr4 pDM8a with full-length apsB3.2 | 35 |
| pDV43 | PTS2 of apsB in pDV21a is mutated alcA(p)::GFP::apsB_PTS2mut pyr4 | This work |
| pDV50 | alcA(p)::YFPC::γtubulin1.8 pyr4 apsB of pDV8 replaced with full-length γtubulin1.8 | This work |
| pENTRMT/D-Topo | Cloning vector | Invitrogen |
| pJH19 | gpd(p)::stuA(NLS)::DsRed argB | 34 |
| pMCB17apx(-apsB) | pMCB17 version for fusion of GFP to N termini of proteins of interest (with 1.5 kb of apsB) | 35; V. P. Efimov |
| pMT-3 × HA | Gateway destination vector | 34 |
| pNZ16 | PTS1 (SRL) added before stop codon of apsB_PTS2mut in pDV43; alcA(p)::GFP::apsB_PTS2mut_SRL pyr4 | This work |
| pNZ17 | pMCB17-apx containing full-length γtubulin of 1.8 kb between AscI and PacI restriction sites; alcA(p)::GFP::γtubulin1.8 pyr4 | This work |
| pNZ21 | apsB3.2 without stop codon in pENTRMT/D-Topo | |
| pNZS23 | apsB3.2 from pNZ21 cloned into pMT-3 × HA alcA(p)::apsB3.2::3 × HA argB | This work |
| pNZ-SI36 | alcA(p) of pNZ17 replaced with 1.16-kb γtubulin(p) EcoRI and BsiwI restriction sites; γtubulin(p)::GFP::γtubulin1.8 pyr4 | This work |
| pNZ-SI37 | alcA(p) of pDV21 Replaced with 1.33-kb apsB(p) AvrII and KpnI restriction sites; apsB(p)::GFP::apsB3.2 pyr4 | This work |
| pRS88 | apsB in BamHI-SalI sites of pGAD424 | This work |
| pRS89 | apsB in BamHI-SalI sites of pGBT9 | This work |
| pRS91 | cDNA clone of hexA in pGAD424 | This work |
| pSI-N4 | pSM14 containing full-length apsB of 3.2 kb between AscI and PacI restriction sites; alcA(p)::3×HA::apsB3.2 pyr4 | This work |
| pSM14 | GFP of pMCB17apx replaced with 3×HA between KpnI and AscI restriction sites | 25 |
| pTN1 | pyroA from A. fumigatus | 23 |