Table 2.

Plasmids used in this study

PlasmidConstructionSource
pCR2.1-TOPOCloning vectorInvitrogen
pDM8aGFP replaced with mRFP1 in pMCB17apx-apsB35
pDV7alcA(p)::YFPN::apsB1.5 pyr4 GFP of pMCB17apx-apsB replaced with YFPNThis work
pDV8alcA(p)::YFPC::apsB1.5 pyr4 GFP of pMCB17apx-apsB replaced with YFPCThis work
pDV15alcA(p)::GFP::hexA680 pyr4 pMCB17apx with full-length hexAThis work
pDV17alcA(p)::YFPC::hexA680 pyr4 apsB of pDV8 replaced with hexA680This work
pDV19alcA(p)::YFPN::hexA1.0 pyr4 apsB of pDV7 replaced with full-length hexA1.0This work
pDV21apMCB17-apx containing full-length apsB of 3.2 kb between AscI and PacI restriction sites; alcA(p)::GFP::apsB3.2 pyr4This work; 35
pDV22balcA(p)::YFPN::apsB3.2 pyr4 apsB of pDV7 replaced with full-length apsB3.2This work
pDV23alcA(p)::YFPC::apsB3.2 pyr4 apsB of pDV8 replaced with full-length apsB3.2This work
pDV39alcA(p)::mRFP1::hexA680 pyr4 apsB of pDM8a changed with hexA68035
pDV42aalcA(p)::mRFP::apsB3.2 pyr4 pDM8a with full-length apsB3.235
pDV43PTS2 of apsB in pDV21a is mutated alcA(p)::GFP::apsB_PTS2mut pyr4This work
pDV50alcA(p)::YFPC::γtubulin1.8 pyr4 apsB of pDV8 replaced with full-length γtubulin1.8This work
pENTRMT/D-TopoCloning vectorInvitrogen
pJH19gpd(p)::stuA(NLS)::DsRed argB34
pMCB17apx(-apsB)pMCB17 version for fusion of GFP to N termini of proteins of interest (with 1.5 kb of apsB)35; V. P. Efimov
pMT-3 × HAGateway destination vector34
pNZ16PTS1 (SRL) added before stop codon of apsB_PTS2mut in pDV43; alcA(p)::GFP::apsB_PTS2mut_SRL pyr4This work
pNZ17pMCB17-apx containing full-length γtubulin of 1.8 kb between AscI and PacI restriction sites; alcA(p)::GFP::γtubulin1.8 pyr4This work
pNZ21apsB3.2 without stop codon in pENTRMT/D-Topo
pNZS23apsB3.2 from pNZ21 cloned into pMT-3 × HA alcA(p)::apsB3.2::3 × HA argBThis work
pNZ-SI36alcA(p) of pNZ17 replaced with 1.16-kb γtubulin(p) EcoRI and BsiwI restriction sites; γtubulin(p)::GFP::γtubulin1.8 pyr4This work
pNZ-SI37alcA(p) of pDV21 Replaced with 1.33-kb apsB(p) AvrII and KpnI restriction sites; apsB(p)::GFP::apsB3.2 pyr4This work
pRS88apsB in BamHI-SalI sites of pGAD424This work
pRS89apsB in BamHI-SalI sites of pGBT9This work
pRS91cDNA clone of hexA in pGAD424This work
pSI-N4pSM14 containing full-length apsB of 3.2 kb between AscI and PacI restriction sites; alcA(p)::3×HA::apsB3.2 pyr4This work
pSM14GFP of pMCB17apx replaced with 3×HA between KpnI and AscI restriction sites25
pTN1pyroA from A. fumigatus23