Table 1.

Strains used in this study

StrainGenotypeLaboratory no.Reference
SC5314Prototrophic clinical isolateDH35 22
CAI4Ura derivative of SC5314 ura3::λimm434/ura3::λimm434DH332 33
CAF2Ura+ derivative of CAI4DH331 33
AH81Ura derivative of CDH107 selected on 5-fluoroorotic acid ura3::λimm434/ura3::λimm434ras1Δ::hisG/ras1Δ::hisGDH482This study
CDH107 (ras1/ras1) ura3::λimm434/ura3::λimm434ras1Δ::hisG-URA3 hisG/ras1Δ::hisGDH483 33
CaAP16 (ras1/ras1/RAS1) ura3::λimm434/ura3::λimm434ras1Δ::hisG/ras1Δ::hisG::FLAG-RAS1-URA3DH1383This study
CR216 (cyr1/cyr1) ura3::λimm434/ura3::λimm434DH346 48
cyr1Δ::hisG-URA3-hisG/cyr1Δ::hisG
RM100 ura3::λimm434/ura3::λimm434DH1270 43
CNC13 (hog1/hog1) ura3::λimm434/ura3::λimm434DH1269 53
his1Δ::hisG/his1Δ::hisG
hog1Δ::hisG-URA3-hisG/hog1Δ::hisG
CU2 (URA3/ura3)Ura+ derivative of CAI4DH1209 40
1F54 (cat1/cat1) ura3::λimm434/ura3::λimm434cat1Δ::hisG/cat1Δ::hisG-URA3DH1213 40
CHK21 (chk1/chk1) ura3::λimm434/ura3::λimm434chk1Δ::hisG-URA3-hisG/chk1Δ::hisGDH177 6
CAT1-GFPCAI4 with plasmid pCAT1-GFP-URA3 integrated at the RPS1 locusaDH939 13
  • a Although the catalase-encoding gene (orf19.6229) has been referred to as CTA1 by Enjalbert et al. (13) and Davis-Hanna et al. (10), we refer to it as CAT1 in accordance with the CGD nomenclature.