Table 1.

Strains used in this study

StrainGenotypeLaboratory no.Reference
SC5314Prototrophic clinical isolateDH3522
CAI4Ura derivative of SC5314 ura3::λimm434/ura3::λimm434DH33233
CAF2Ura+ derivative of CAI4DH33133
AH81Ura derivative of CDH107 selected on 5-fluoroorotic acid ura3::λimm434/ura3::λimm434 ras1Δ::hisG/ras1Δ::hisGDH482This study
CDH107 (ras1/ras1)ura3::λimm434/ura3::λimm434 ras1Δ::hisG-URA3 hisG/ras1Δ::hisGDH48333
CaAP16 (ras1/ras1/RAS1)ura3::λimm434/ura3::λimm434 ras1Δ::hisG/ras1Δ::hisG::FLAG-RAS1-URA3DH1383This study
CR216 (cyr1/cyr1)ura3::λimm434/ura3::λimm434DH34648
cyr1Δ::hisG-URA3-hisG/cyr1Δ::hisG
RM100ura3::λimm434/ura3::λimm434DH127043
CNC13 (hog1/hog1)ura3::λimm434/ura3::λimm434DH126953
his1Δ::hisG/his1Δ::hisG
hog1Δ::hisG-URA3-hisG/hog1Δ::hisG
CU2 (URA3/ura3)Ura+ derivative of CAI4DH120940
1F54 (cat1/cat1)ura3::λimm434/ura3::λimm434 cat1Δ::hisG/cat1Δ::hisG-URA3DH121340
CHK21 (chk1/chk1)ura3::λimm434/ura3::λimm434 chk1Δ::hisG-URA3-hisG/chk1Δ::hisGDH1776
CAT1-GFPCAI4 with plasmid pCAT1-GFP-URA3 integrated at the RPS1 locusaDH93913
  • a Although the catalase-encoding gene (orf19.6229) has been referred to as CTA1 by Enjalbert et al. (13) and Davis-Hanna et al. (10), we refer to it as CAT1 in accordance with the CGD nomenclature.