TABLE 2.

Oligonucleotides

NameSequencea (5′-3′)Site added
BNI128N forCCCGGTACCTTGTGCCAGAAGAATACGCCAAAGTATTAATGTCAKpnI
BNI128N revCCTGGGCCCTTTTTTTCGTGTGATAATTCTTGGTGATAATAAApaI
BNI128C forCCTCCGCGGTTGGTGGTGTTATGGATTCATTATTACAGAGGTTGSacII
BNI128C revCCTGAGCTCTTTCTCTGGATCTTCTACATTACTGAAATCSacI
BNI518N forGGTGGTACCTTGTATCACAAACATCTTTTCATCATCCACTACTAKpnI
BNI518N revGGTGGGCCCGGTTCTTTTATTTATTTATTTTTTApaI
BNI518C forGGTCCGCGGGGTAAGCACTTTTTTTTGTATATGTATTTAAATGASacII
BNI518C revGGTGAGCTCGGTTGTTATCACTAAATAAACCAAAATTTGAATACSacI
NAT1 forTTCGAGCTCGTTTAAACGTCGACACTGGATGGSacI
NAT1 revCAGTAGCGCCAGCGGAAGAGCAACGGGATATCAAGCTTGCCTCGTCCCSapI
MEA366 forCCCGGTACCCACGCACTAACACTTAAACACACGCCCKpnI
MEA366 revAGGGGATCCGTACAAGACGTCCAGGTGATAGATTATBamHI
MEA500N forGGTGGTACCTTTTAATAGTATTGAGTTATCCAAGAGAGTAGTGAKpnI
MEA500N revCCGGGGCCCAAGTTATGAAGGTATCCGTTTTTATGTCCAATAGAApaI
MEA500C forGGTCCGCGGGTGAATTTGGTTTATTATAGTTTTGGTTTGATTCTSacII
MEA500C revGGTGAGCTCGGTAAATCAAAATATGTTGGTTCTGGTGSacI
MEA700N forGGTGGTACCTTTCGCACTAACACTTAAACACACGCCCATKpnI
MEA700N revGGTGGGCCCGTACTTTTTGGTTGTTACTATATGTAATApaI
MEA700C forGGTCCGCGGGGTACTAGGGGTATAATACTTACTTTCTTCAACTTSacII
MEA700C revGGTGAGCTCGGTGAATATTTGGATCAATAGCCATTGGATTASacI
  • a Engineered restriction sites are in boldface.