TABLE 3.

Phenotypes conferred on S. cerevisiae AD1-8u by hyperexpression of fungal drug efflux pumps and other membrane proteins

Hyperexpressed proteinMIC of drug (μg/ml)R6G effluxa (pmol/107 cells/min)ATPase activitya,b (nmol Pi/min/mg protein)
FLCITCNYS
None (pABC3)0.50.03120.07 ± 2
ScPdr5p300>32293 ± 5199 ± 12
CaCdr1Ap (allele A)200>32275 ± 9185 ± 4
CaCdr1Bp (allele B)300>32279 ± 7188 ± 3
CaCdr2Ap (allele A)754222 ± 5128 ± 9c
CaCdr2Bp (allele B)150>32233 ± 3164 ± 16c
CaMdr1Ap600.03120.06 ± 2
CaErg11Ap50.12520.05 ± 2
CgCdr1p300>324105 ± 1200 ± 14
CgPdh1p80.062415 ± 3141 ± 19
CkAbc1p328282 ± 557 ± 2
CneMdr1p5>32292 ± 2045 ± 3
  • a Mean ± SD for three experiments carried out in triplicate.

  • b Oligomycin (20 μM)-sensitive ATPase activity, measured at pH 7.5.

  • c Two hundred micromolar oligomycin used in assays.