TABLE 1.

Strains of S. cerevisiae used in this study

StrainGenotypeReference or source
SGY398MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP1 bcy1Δ::LEU253
SGY446MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP144
SGY448MATa ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::URA3 tpk2-63(Ts) tpk3::TRP144
SGY562MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-62(Ts) tpk3::TRP1This study
SGY559SGY446 TPK2This study
NOY16MATα/MATa ura3-52/ura3-52 his3/his3 leu2-3,112/leu2-3,112 trp1/trp1 ade8/ade8 tpk1::ADE8/tpk1::URA3 tpk2-63(Ts)/tpk2-63(Ts) tpk3::TRP1/tpk3::TRP1This study
SGY530MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP1 CDC37-101This study
SGY533MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP1 CDC37-106This study
SGY535MATa ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP1 CDC37-109This study
MRY140SGY562(pRS316)This study
MRY141SGY562(pRS316-CDC37-101)This study
FY70MATα ura3-52 his3 leu2-3,112 trp1 ade1 TPK2
MRY142FY70 ura3::pRS306-tpk2-63-myc12This study
MRY143FY70 ura3::pRS306-TPK2-myc12This study
MRY31SGY559(pRS316)[pADH-PKI-MSN2-(576-704)-GFP3]This study
MRY43SGY562(pRS316)[pADH-PKI-MSN2-(576-704)-GFP3]This study
MRY45SGY562[pRS316-CDC37-101] [pADH-PKI-MSN2-(576-704)-GFP3]This study
MRY4MATα cdc37Δ::KanMx lys2 (pRS202-CDC37+)This study
MRY5MATα cdc37Δ::KanMx lys2 (pRS316-CDC37-109)This study
MRY8MATα cdc37Δ::KanMx met15 (pRS316-CDC37+)This study
MRY11MATα cdc37Δ::KanMx lys2 (pRS316-CDC37-101)This study
MRY10BRY4742(pRS316-cdc37Δ)This study
MRY47SGY562 sti1-1::HIS3 (pRS316-CDC37-101)This study
MRY73MATα cdc37Δ::KanMx his3Δ1 leu2Δ1 met15Δ0 ura3Δ1 (pRS315-CDC37+)This study
MRY75MATα cdc37Δ::KanMx his3Δ1 leu2Δ1 met15Δ0 ura3Δ1 (pRS315-CDC37-106)This study
MRY79MRY73(His6-V5-Ste11ΔN)This study
MRY81MRY75(His6-V5-Ste11ΔN)This study
ACY77-3MATα ade2 leu2 his3 trp1 ura3 sti1-1::HIS3This study
Y703MATα cdc25-1 ura3-52 leu2-3,112 trp1 ade1Jim Broach
WX241-12bMATα mps1-1 ura3-52 his3Δ20042