TABLE 1.

Effects of zinc status on the accumulation and distribution of zinca

StrainTreatmentZn content (nmol/mg [dry wt]) (mean ± SE)CompartmentP value
Whole cellVacuoleCytoplasmL vs MM vs HL vs H
WTLND1.4 ± 1.71.7 ± 1.6Whole cellNS<0.001<0.005
MNDNDNDVacuoleNS<0.0001<0.0001
H3.8 ± 0.911.9 ± 1.40.7 ± 0.9CytoplasmNSNSNS
0 vs 1010 vs 200 vs 20
WT0ND1.4 ± 1.71.7 ± 1.6Whole cell<0.0001NS<0.0001
107.1 ± 1.018.6 ± 2.41.4 ± 1.2Vacuole<0.0001<0.05<0.0001
209.2 ± 1.736.4 ± 5.33.4 ± 1.0CytoplasmNSNSNS
zrc1Δ cot1Δ mutant0ND0.2 ± 1.2NDWhole cell<0.0001<0.01<0.0001
101.9 ± 0.66.5 ± 1.30.7 ± 0.9Vacuole<0.0001<0.001<0.0001
205.6 ± 1.013.9 ± 2.61.8 ± 0.7CytoplasmNSNSNS
  • a The levels of zinc in whole cells, vacuoles, and the nonvacuolar cytoplasm were determined by EPXMA under steady-state (first three rows) and zinc shock (all other rows) conditions. Data were obtained from 17 to 76 cells for whole-cell measurements, 12 to 44 cells for vacuole measurements, and 17 to 43 cells for nonvacuolar cytoplasm measurements, depending on the strain and growth condition. WT, wild type; L, low zinc (CSD medium); M, moderate zinc (SD medium with 0 Zn); H, high zinc (SD medium plus 100 μM Zn); 0, 0 min; 10, 10-min Zn treatment; 20, 10-min Zn treatment plus 20-min chase; ND, not detectable; NS, not significant (P > 0.05).