TABLE 2.

Effects of zinc status on the accumulation and distribution of other elements in wild-type cellsa

ElementTreatmentElement content (nmol/mg [dry wt]) (mean ± SE)CompartmentP value
Whole cellVacuoleCytoplasmL vs MM vs HL vs H
CaL15.8 ± 0.928.4 ± 2.012.3 ± 0.9Whole cell<0.005<0.0001NS
M12.1 ± 0.918.9 ± 2.29.9 ± 1.1Vacuole<0.001NS<0.05
H16.8 ± 0.723.2 ± 2.114.2 ± 0.7CytoplasmNS<0.0001NS
ClL62.4 ± 7.753.9 ± 7.181.7 ± 15.4Whole cellNS<0.0001<0.0001
M68.5 ± 6.635.6 ± 4.281.8 ± 9.1Vacuole<0.05<0.0001<0.0001
H11.2 ± 0.67.8 ± 0.612.0 ± 0.6CytoplasmNS<0.0001<0.0001
FeL3.0 ± 0.22.4 ± 0.12.8 ± 0.2Whole cellNSNSNS
M4.2 ± 0.73.5 ± 0.94.2 ± 1.0VacuoleNSNSNS
H3.1 ± 0.12.9 ± 0.13.1 ± 0.2CytoplasmNSNSNS
KL511.6 ± 21.0266.8 ± 20.6664.8 ± 23.5Whole cellNS<0.0001<0.0001
M557.1 ± 24.2539.6 ± 53.3525.8 ± 26.5Vacuole<0.0001<0.0001<0.0001
H806.9 ± 23.5752.2 ± 34.9761.1 ± 33.2Cytoplasm<0.005<0.0001NS
MgL82.7 ± 9.4236.4 ± 19.042.7 ± 1.9Whole cellNS<0.0001<0.0001
M82.3 ± 8.0223.3 ± 17.046.6 ± 4.6VacuoleNS<0.005<0.05
H150.9 ± 11.2320.7 ± 18.993.5 ± 3.5CytoplasmNS<0.0001<0.0001
NaL11.7 ± 6.316.8 ± 11.914.9 ± 13.1Whole cell<0.0001<0.0001NS
MNDNDNDVacuole<0.0001<0.0001<0.0001
H9.8 ± 2.637.4 ± 4.30.6 ± 2.6Cytoplasm<0.0001<0.0001NS
PL670.4 ± 49.21,496.6 ± 68.1447.9 ± 12.6Whole cellNS<0.0001<0.0001
M645.3 ± 50.91,422.8 ± 83.8449.9 ± 42.9VacuoleNS<0.0001<0.001
H1,084.2 ± 48.61,812.7 ± 65.1830.3 ± 20.4CytoplasmNS<0.0001<0.0001
0 vs 1010 vs 200 vs 20
Ca015.8 ± 0.928.4 ± 2.012.3 ± 0.9Whole cellNS<0.0001<0.005
1018.1 ± 0.920.9 ± 2.216.8 ± 1.1Vacuole<0.0001<0.0001<0.0001
2012.4 ± 0.412.2 ± 0.512.7 ± 0.4Cytoplasm<0.005<0.05NS
Cl062.4 ± 7.753.9 ± 7.181.7 ± 15.4Whole cell<0.0001<0.0001NS
1035.5 ± 4.929.3 ± 4.253.7 ± 9.7Vacuole<0.001NS<0.005
20101.6 ± 16.038.6 ± 8.693.3 ± 19.1Cytoplasm<0.001<0.01NS
Fe03.0 ± 0.22.4 ± 0.12.8 ± 0.2Whole cellNS<0.05<0.001
102.7 ± 0.12.3 ± 0.22.9 ± 0.2VacuoleNSNSNS
202.4 ± 0.22.4 ± 0.32.1 ± 0.2CytoplasmNS<0.005<0.05
K0511.6 ± 21.0266.8 ± 20.6664.8 ± 23.5Whole cellNS<0.005<0.005
10527.9 ± 19.7506.6 ± 34.0507.5 ± 11.8Vacuole<0.0001NS<0.0001
20634.6 ± 31.7452.1 ± 26.2680.7 ± 36.0Cytoplasm<0.0001<0.0001NS
Mg082.7 ± 9.4236.4 ± 19.042.7 ± 1.9Whole cellNSNSNS
1081.2 ± 6.3196.5 ± 10.747.9 ± 2.2VacuoleNSNS<0.05
2071.5 ± 5.5189.9 ± 9.844.8 ± 4.0CytoplasmNS<0.05NS
Na011.7 ± 6.316.8 ± 11.914.9 ± 13.1Whole cellNS<0.001NS
109.5 ± 4.446.9 ± 10.72.9 ± 3.6Vacuole<0.05<0.01<0.0001
2016.9 ± 3.679.1 ± 12.72.1 ± 1.2CytoplasmNSNSNS
P0670.4 ± 49.21,496.6 ± 68.1447.9 ± 12.6Whole cellNSNSNS
10615.0 ± 35.51,274.9 ± 31.9407.8 ± 11.8Vacuole<0.05NS<0.005
20607.5 ± 40.71,240.2 ± 64.2431.3 ± 41.4CytoplasmNSNS<0.05
  • a The levels of the listed elements in whole cells, vacuoles, and the nonvacuolar cytoplasm were determined by EPXMA under steady-state (first set of elements Ca to P) and zinc shock (second set of elements Ca to P) conditions. Data were obtained from 34 to 76 cells for whole-cell measurements, 17 to 44 cells for vacuole measurements, and 17 to 43 cells for nonvacuolar cytoplasm measurements, depending on the growth condition. See footnote a of Table 1 for abbreviations.