TABLE 2.

Plasmids used in this study

PlasmidDescriptionSource
pABC3Empty expression vectorThis study
pABC3-HISVector pABC3 containing a C-term. hexahistidine affinity tagThis study
pABC3-CYSpABC3 containing a C-terminal tetracysteine tagThis study
pABC3-HIS-CYSpABC3 containing a C-terminal tandem tag (hexa-histidine and tetra-cysteine)This study
pABC3-FLAG-HISpABC3 containing a C-terminal tandem tag (FLAG and hexa-histidine tag)This study
pABC3-GFPpABC3 containing C-terminal YEGFP3This study
pABC3-mRFPpABC3 containing C-terminal mRFP1This study
pABC5'Identical to pABC3 but contains HIS1 instead of URA3This study
pABC5′-HISIdentical to pABC3-HIS but contains HIS1 instead of URA3This study
pABC5′-CYSIdentical to pABC3-CYS but contains HIS1 instead of URA3This study
pABC5′-HIS-CYSIdentical to pABC3-HIS-CYS but contains HIS1 instead of URA3This study
pABC5′-FLAG-HISIdentical to pABC3-FLAG-HIS but contains HIS1 instead of URA3This study
pABC5′-GFPIdentical to pABC3-GFP but contais HIS1 instead of URA3This study
pABC5′-mRFPIdentical to pABC3-mRFP but contains HIS1 instead of URA3This study
pABC5′-ScPDR5pABC3 with PDR5 ORF from Saccharomyces cerevisiae AD1-8uThis study
pABC3-CaCDR1ApABC3 with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-HISpABC3-HIS with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-CYSpABC3-CYS with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-HIS-CYSpABC3-HIS-CYS with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-FLAG-HISpABC3-FLAG-HIS with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-GFPpABC3-GFP with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1A-mRFPpABC3-mRFP with A allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR1BpABC3 with B allele of CDR1 ORF from Candida albicans strain 10261This study
pABC3-CaCDR2ApABC3 with A allele of CDR2 ORF from Candida albicans strain 10261This study
pABC3-CaMDR1ApABC3 with A allele of MDR1 ORF from Candida albicans strain 10261This study
pABC3-CaERG11ApABC3 with A allele of ERG11 ORF from Candida albicans strain 10261This study
pABC3-CneMDR1pABC3 with MDR1 ORF from Cryptococcus neoformans strain CDC551This study
pABC3-HsABCB1pABC3 with HsABCB1 ORF from Homo sapiensThis study