TABLE 4.

Effect of tags on pump ATPase activity and response to inhibitors

Hyperexpressed pumpKinetics of ATPase activityIC50 of:
Km app (mM ATP)Vmax (nmol/min/mg)pH optimumVanadate (μM)Oligomycin (μM)FK506 (μg/ml)
None (AD1-8u)2.61306-8
ScPDR51.423037.51.30.250.013
ScPDR5-His1.303337.51.30.300.013
CaCDR1A1.311357.53.10.300.63
CaCDR1A-His1.201337.53.10.300.63
CaCDR1A-Cys1.821117.5-8.012.50.700.80
CaCDR1A-His-Cys1.452047.56.30.700.60
CaCDR2A2.452507.51.3>2020
CaCDR2A-His1.052787.53.0>2020
CgCDR11.952277.51.50.0630.0063
CgCDR1-His1.273037.51.60.010.013
CgPDH10.602707.00.90.300.013
CgPDH1-His0.853037.01.30.300.013
CkABC11.311617.50.80.401.0
CkABC1-His1.452047.51.30.401.0
CneMDR11.71956.5-7.03.10.5020
CneMDR1-His1.03796.5-7.04.01.0020