TABLE 1.

MYC3-tagged pacC alleles and strains constructed and characterized in this worka

AlleleChange(s) in proteinNucleotide change(s)Strain nameGenotypeResult by Western analysis
pacC900(L481A)L481AC2399G T2400CMP87 pacC900(L481A)+
pacC900(G482A)G482AG2403CMP123 pacC900(G482A)
pacC900(F485A)F485AT2411G T2412CMP91 yA2 pacC900(F485A)+
pacC900(D486A)D486AA2415CMP114 yA2 pabaA1 pacC900(D486A)+
pacC900(D488A)D488AA2421CMP84 yA2 inoB2 pacC900(D488A)+
pacC900(E489A)E489AA2424CMP160 yA2 pabaA1 inoB2 pacC900(E489A)+
pacC900(R490A)R490AC2426G G2427CMP82 pacC900(R490A)
pacC900(R491A)R491AC2429G G2430CMP79 pacC900(R491A)+
pacC900(R491A Q499A)R491AC2429G G2430CMP116 inoB2 pacC900(R491A Q499A)+
Q499AC2453G A2454C
pacC900(R492A)R492AA2432G G2433CMP121 yA2 pabaA1 pacC900(R492A)
pacC900(Y493A)Y493AT2435G A2436CMP104 inoB2 pacC900(Y493A)+
pacC900(T494A)T494AA2438GMP94 yA2 pabaA1 pacC900(T494A)+
pacC900(G495A)G495AG2442CMP100 pacC900(G495A)+
pacC900(G496A)G496AG2445CMP98 pacC900(G496A)
pacC900(L498A)L498AT2450GMP190 adE20 pyroA4 pacC900(L498A)+
pacC900(L498F)L498FG2452CMP212 adE20 pacC900(L498F)+
pacC900(L498S)L498ST2451CMP176 adE20 pacC900(L498S)+
pacC900(Q499A)Q499AC2453G A2454CMP76 inoB2 pacC900(Q499A)+
pacC900 NoneNone yA2 pabaA1 pacC900 +
  • a Amino acids and nucleotides are as described by Tilburn et al. (69).