TABLE 2.

palB mutations characterized in this worka

AlleleNucleotide changeChange in proteinGrowth at pH 8.0dMutagen methodSelectionReference
20°C37°C
Wild typeNoneNone++++++++++NoneNone 8, 67
Truncating mutations
    palB508T340GL13stopUVDiploid GABAThis work
    palB510T340AL13stopUVDiploid GABAThis work
    palB70A359TPSDNDNoneHaploid GABAE. A. Espeso (unpublished)
    palB38A515TK55stopUVHaploid GABAThis work
    palB509del(658-662)R103fscUVDiploid GABAThis work
    palB502T678GL109stopUVDiploid GABAThis work
    palB501C680TQ110stopUVDiploid GABAThis work
    palB518T849CPSD+++/−UVDiploid GABAThis work
    palB520A866TK144stopUVDiploid GABAThis work
    palB519A1638TK385stopUVDiploid GABAThis work
    palB500G1767TE428stopUVDiploid GABAThis work
    palB505delA2007K508fsUVDiploid GABAThis work
    palB513T2140GL552stopUVDiploid GABAThis work
    palB511delG2801 A2803GY773fsUVDiploid GABAThis work
    palB111G2784TE767stopNTGHaploid GABAThis work
    palB7G2856TG791stop+/−UVLack of alkaline phosphatase 18
    palB11bdel(G2863-C2873)R793fsUVLack of alkaline phosphatase 18
    palB37G2925TE814stop+/−UVHaploid GABAThis work
Missense mutations
    palB517T1027G G1029TD197E C198FUVDiploid GABAThis work
    palB16GT(1034 1035)AAV200NUVHaploid GABAThis work
    palB504T1405GL307R+UVDiploid GABAThis work
    palB524T2388AI618L+++++++UVDiploid GABAThis work
    palB39T2340CS619P+++UVHaploid GABAThis work
    palB515T2713CL743P++UVDiploid GABAThis work
    palB516A2778C T2783CT765P++UVDiploid GABAThis work
    palB503T2875CF797S++++++UVDiploid GABAThis work
    palB514T2923CL813P++UVDiploid GABAThis work
  • a Nucleotides and amino acids are numbered as in GenBank Z54244. PSD, predicted splicing defect; ND, not determined; NTG, N-methyl-N′-nitro-N-nitrosoguanidine.

  • b Formerly palE11; see the work of Arst et al. (1). The palB11 phenotype, which is more extreme than that of the more N-terminal nonsense palB7 mutation, is possibly influenced by 17 out-of-frame residues at the C terminus of the protein.

  • c fs, frameshift.

  • d −, no growth; +/−, very small amount of growth; + to +++++, increasing amounts of growth from small to wild-type.