TABLE 2.

Novel E. histolytica introns culled from the EST dataa

Representative ESTTranscriptGenBank accession no.Predicted function5′ Splicing site3′ Splicing siteBLAT coordinatesBLAT scaffoldSizeNo. of unique ESTsEffect on protein
Class I
    EHAA254TF101.m00114ELA47515.1Conserved hypothetical proteinGUUUGUAAG10427-10489101621Alters the C terminus
    EHAA741TF42.m00181N/APseudogene Ras family GTPaseGUUUGUUAG30857-30960421037Alters the C terminus
    EHAF244TR19.m00316ELA50600.1Hypothetical proteinGUUUGUUAG77147-7720719601Eliminates amino acids
    EHAA453TR178.m00101ELA45803.13′ UTR of hypothetical proteinGUUUGUAAG36163-36214178513Alters the C terminus
    EHAA547TR110.m00129ELA47260.1Rho family GTPaseGUUUGUUAG38325-38392110672Alters the C terminus
    EHABP41TR254.m00073ELA44597.1Hypothetical proteinGUUUGUAAG5957-6014254573Alters the C terminus
    EHADQ25TR18.m00335ELA50675.1DNA replication licensing factorGUUUGUUAG98259-9834318841Alters the C terminus
    EHAET77TR18.m00328ELA50668.1Molybdopterin biosynthesisGUUUGUUAG90251-9031218612Alters the C terminus
    EHAGK16TR264.m00090ELA44495.1Sec13 proteinGUAUGUUAG5564-5620264563Alters the N terminus
    EHAH331TR264.m00090ELA44495.1Sec13 proteinGUUUGUUAG5656-5710264542Alters the N terminus
    EHAE226TR133.m00132N/AHypothetical proteinGUUUGUUAG16335-16395133601Alters the N and C termini
    EHAB255TR52.m00167ELA49102.1Rho family GTPaseGUUUGUUAG69361-6942452635Alters the C terminus
    EHAA702TR231.m00059ELA44885.1Conserved hypothetical proteinGUUUGUAAG7731-78342311038Alters the N terminus
    EHAG185TRb47.m00184ELA49297.1Hypothetical proteinGUUUGUAAG70138-7019547571Alters the N terminus
    EHABT01TR57.m00155ELA48912.1Hypothetical proteinGUUCGUUAG34651-3470257511Eliminates amino acids
    EHAE044TR364.m00046ELA43561.160S ribosomal protein L27aGUUUGUUAG15314-154293641151Alters the N terminus
    EHAET36TR135.m00095ELA46630.1Hypothetical proteinGUUUGUUAG9119-92591351406Alters the N terminus
    EHADY14TR366.m00044ELA43555.1Hypothetical proteinGUUUGUUAG6738-6786366481Alters the N terminus
    EHAG990TR152.m00113ELA46298.1Hypothetical proteinGUUUGUUAG24791-249271521361Alters the N terminus
    EHAAP93TR195.m00094ELA45475.160S ribosomal protein L24GUUUGUUAG31405-31461195562Alters the N terminus
Class II
    EHAAY54TR88.m00175ELA47893.13′ UTR of hypothetical proteinGUUUGUUAG55917-56020881032N/A
    EHAE226TR23.m00311ELA50387.13′ UTR of in Rho GTPaseGUUUGUUAG24514-2457423601N/A
    EHAES83TR350.m00049ELA43647.15′ UTR of in Rho GTPaseGUUAAGUAG5995-61433501481N/A
    EHAA378TRb21.m00231ELA50513.15′ UTR of 40S ribosomal protein S14GUUUGUUAG17466-1753921733N/A
    EHAA726TR312.m00035ELA43981.15′ UTR of 60S ribosomal protein L9GUUUGUUAG6822-695631213412N/A
    EHAHG49TR144.m00101ELA46471.1Similar to cap binding proteinGUUUGUUAG16107-16176144691N/A
    EHAF084TR39.m00252ELA49583.1GlycotransferaseGUUUGAUAG80547-8060239551N/A
Class III
    EHAAM93TRN/AN/ASimilar to 6.m00429GUUUGAUAG14120-14172338521New to E. histolytica
    EHACJ50TRbN/AN/ASimilar to pantothenate kinaseGUUUGUAAG76031-7610739761New to E. histolytica
    EHAFD09TRN/AN/ASimilar to UFD1-1GUUUGUUAG73812-7385911471New to E. histolytica
    EHAC353TRbN/AN/ASimilar to acriflavin resistance proteinGUUUGUUAG4398-4451389532New to E. histolytica
    EHAEL21TRN/AN/ASimilar to YIP1 Golgi proteinGUUUGUUAG52191-5224062492New to E. histolytica
    EHAEU30TRN/AN/ACCCH-domain proteinGUUAGUUAG89669-897345652New to E. histolytica
    EHACJ32TRN/AN/ACCCH domain proteinGUUUGUUAG90154-902205662New to E. histolytica
    EHAHB45TRbN/AN/ANo homology, novelGUUUGUUAG23491-23547154561New to E. histolytica
  • a Class I introns align by BLAT to genes that were not annotated to contain an intron in that region. Class II introns align by BLAT to the UTRs of genes. Class III introns align by BLAT to regions that were not annotated to contain genes. N/A, not applicable.

  • b EST for which the spliced product has been cloned and sequenced.