TABLE 1.

Strains used in this study

StrainGenotypeSource
CD16-1 h + /h ade6-M210/ade6-M216 cyh1/+ +/lys5-391C. Shimoda
CD16-5 h /hade6-M210/ade6-M216 cyh1/+ +/lys5-391C. Shimoda
TP4-5A h ade6-M210 leu1-32 ura4-D18 C. Shimoda
TP4-1D h + ade6-M216 his2 leu1-32 ura4-D18 C. Shimoda
AO24 h ade6-M210 leu1-32 ura4-D18 mcp4::ura4+This study
AO28 h + ade6-M216 his2 leu1-32 ura4-D18 mcp4::ura4+This study
AO89 h /hade6-M210/ade6-M216 ura4-D18/ura4-D18 leu1-32/leu1-32 pat1-114/pat1-114 mcp3::[mcp3+-9myc-3′UTR-LEU2]/mcp3::[mcp3+-9myc-3UTR-LEU2] mcp4::[mcp4-3ha-3UTR-ura4+]/mcp4::[mcp4+-3ha-3′UTR-ura4+]This study
AO64 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+]This study
AO00 h 90 leu1-32 pRGT41-mcp4+This study
YN68 h 90 leu1::[gfp-psy1+-leu1+]C. Shimoda
AO185 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+] meu14::ura4+This study
AO187 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+] meu14::meu14+-gfpThis study
AO192 h 90 ade6-M210 leu1-32 ura4-D18 (or +) mcp4::[mcp4-3ha-3′UTR-ura4+] crn1+-gfp::kanMX6This study
AO189 h 90 leu1-32 ura4-D18 mcp4::ura4+ pRGT41-mcp4+This study
AO190 h 90 leu1-32 ura4-D18 mcp4::ura4+ pRGT41-mcp4ΔCThis study
AO125 h 90 leu1::gfp-psy1+ura4-D18 (or +) mcp4::ura4+This study
AO181 h 90 leu1-32 pRGT41-mcp4ΔCThis study
FY13596a h 90 crn1 +-gfp::Kanrade6-M210 leu1-32YGRC/NBRP
AO168 h 90 ade6-M210 leu1-32 pREP1-rer1+-cfp ura4-D18 pRGT2-mcp4ΔCThis study
AO165 h + ade6-M216 leu1-32 his2 ura4-D18 lys3 cdc12 mcp4::ura4+This study
AO162 h ade6-M210 ura4-D18 mcp4::ura4+This study
AO194 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+] pREP81-gfp-psy1This study
AO196 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+] spn6-gfp::KanrThis study
AO200 h 90 leu1-32 ura4-D18 mcp4::[mcp4+-3ha-3′UTR-ura4+] cut15-gfp::LEU2This study
ST272 h + ade6-M210 his2 leu1-32 ura4-D18 lys3 cdc12 Our stock
NP16-6B h ade6-M216 ura4-D18 Our stock
AO54 h ura4-D18 mcp4::ura4+This study
AO122 h + his2 leu1-32 ura4-D18 lys3 cdc12 mcp4::ura4+This study
MS105-1B h ade6-M26 ura4-D18 Our stock
MS111w1 h + ade6-469 ura4-D18 leu1-32 his2 Our stock
TT8-1 h ura4 + Our stock
NP32-2A h + leu1-32 his2 ura4-D18 Our stock
AO127 h ade6-M26 ura4-D18 mcp4::ura4+This study
AO128 h + his2 ade6-469 ura4-D18 mcp4::ura4+This study
JZ670 h /hade6-M210/ade6-M216 ura4-D18/ura4-D18 leu1-32/leu1-32 pat1-114/pat1-114M. Yamamoto
AO115 h /hade6-M210/ade6-M216 ura4-D18/ura4-D18 leu1-32/leu1-32 pat1-114/pat1-114 mcp4::ura4+/mcp4::ura4+This study
AO56 h 90 ade6-M210 leu1-32 ura4-D18 mcp4::[mcp4+-gfp-3′UTR-ura4+]Our stock
  • a This strain was obtained from the Yeast Genetic Resource Center of Japan, supported by the National BioResource Project (YGRC/NBRP [http://yeast.lab.nig.ac.jp/nig/ ]).