TABLE 1.

E. histolytica intron splice site misannotationsa

Representative ESTTranscriptGenBank accession no.Predicted functionPredicted sequence at indicated splicing site:Predicted coordinatesPredicted sizeBLAT-EST sequence at indicated splicing site:BLAT-EST coordinatesBLAT- EST sizeNo. of unique ESTs
5′3′5′3′
EHAG374TR6.m00476ELA51573.1Hypothetical proteinGUAUUUGAG122713-123030317GUUUGUUAG122735-122797621
EHAA018TR18.m00295ELA50710.1Hypothetical proteinGUUUAUAAG16364-16517153GUUUGUUAG16351-1646311266
EHAAB92TF88.m00175ELA47893.1Hypothetical proteinGUAUUGGAG56505-57006501GUUUGUUAG56507-5668117410
EHAE529TR50.m00171ELA49178.1Vesicular transportGUUGGAUAG22303-2239087GUUUGUUAG22293-22368751
EHAC267TR42.m00212ELA49475.1S6 Ribosomal proteinGUUAUGUAG76818-7688062GUAUGUUAG76814-76870563
EHAC267TR42.m00214ELA49453.1S6 Ribosomal proteinGUUAUGUAG86245-8630762GUAUGUUAG86241-86296563
EHAC267TR1066.m00011ELA42451.1S6 Ribosomal proteinGUCCAUAAG270-36595GUAUGUUAG280-335563
EHAES83TR350.m00049ELA43647.1Rab family GTPaseGUAAUUUAG6630-667950GUUUGUUAG6625-6679543
EHAES83TR350.m00049ELA43647.1Rab family GTPaseGUUUGUUAG6442-6562121GUUUGUUAG6442-6503613
EHAES83TR350.m00049ELA43647.1Rab family GTPaseGUUUGUUAG6442-6562121GUUUGUUAG6513-6563503
  • a Data for eight intron-containing genes for which the intron prediction did not match the EST data are shown.