TABLE 3.

Genes for synthesis of lipids in the C. merolae genome

SubstrateProductEnzyme or gene nameLocus tag(s) and/or gene name(s)a
NANAACPacpP (cp)
Acetyl-CoAMolonyl-CoAAcetyl-CoA carboxylase (AccABCD)accABD (cp), accC (cp), CMS299C*
Acetyl-CoAMolonyl-CoAAcetyl-CoA carboxylase (single subunit type)CMM188C
Molonyl-CoAMolonyl ACPTransacylaseCMT420C
Molonyl ACPAcyl ACPFatty acid synthase complexCMM286C*/CML329C (cytosol), CMS393C*, CMI240C, CMT381C*
Acyl ACPFatty acidThioesteraseNone
Fatty acidAcyl CoAAcyl-CoA synthetaseCME186C
Acyl-CoA1-LPAG3P acyltransferaseCMJ027C
1-LPAPhosphatidic acidLPA acyltransferaseCMJ021C, CMF185C, CME109C
DiacylglycerolPhosphatidic acidDG kinaseCMR054C
DiacylglycerolMGDGMGDG synthaseCMI271C*
DiacylglycerolGlcDGSll1377 (Synechocystis)CMT267C?b
DiacylglycerolSQDGSQD1/SQD2CMR012C/CMR015C
Ethanolamine phosphateCDP ethanolamineMUQ1CMS052C
DiacylglycerolPEEthanolamine phosphotransferase (EPT1)CMF133C
DiacylglycerolTriacylglycerolDG acyltransferaseCMQ199C
PEPCMethyltransferasesCMF090C/CMA134C
Phosphatidic acidCDP-DGCDS1CMN215C
CDP-DGPGPPGS1CMJ134C
PGPPGUnknownUnknown
CDP-DGPIPIS1CMM125C
MGDGDGDGDGD1/DGD2None
MGDGDGDGDGDG synthaseycf82 (cp)?c
PGDPGCLSCMN196C?d
Stearyl ACPOleoyl ACPStearoyl ACP desaturaseNone
Stearoyl-CoAOleoyl-CoAStearoyl-CoA desaturase (cytochrome b fusion)CMM045Ce (ER)
Stearoyl lipid (?)Oleoyl lipid (?)Stearoyl lipid (?) desaturaseCMJ201C (particulate)
Oleoyl lipid (?)Linoleoyl lipid (?)Oleoyl lipid (?) desaturaseCMK291C (ER)
Farnesyl diphosphateSqualeneSqualene synthaseCMG178C
Squalene2,3-EpoxysqualeneSqualene monooxygenaseCMH256C
2,3-EpoxysqualeneLanosterolLanosterol synthaseCMJ009C
  • a cp, genes carried by the chloroplast genome. Genes were identified by sequence similarity and annotations are included in the Cyanidioschyzon Genome Project (28), except where relevant references are given. Asterisks indicate that the plastid localization was confirmed by GFP experiments, while other localizations are shown in parentheses.

  • b See reference 6.

  • c Sakurai, Mizusawa, Wada, and Sato, unpublished data.

  • d See reference 21.

  • e See reference 20.