TABLE 1.

Strains and plasmids

Strain or plasmidGenotype or descriptionReference or source
Strains
    SC5314Wild type14
    CAI4Δura3::imm434/Δura3::imm43412
    HB-12As CAI4 but with plasmid pHWP1GFP3 integrated at the HWP1 locusThis study
    EGFP3As CAI4 but with plasmid pENO1GFP3 integrated at the ENO1 locus39
    −2025As CAI4 but with plasmid p2025 integrated at the ENO1 locusThis study
    −1902As CAI4 but with plasmid p1902 integrated at the ENO1 locusThis study
    −1782As CAI4 but with plasmid p1782 integrated at the ENO1 locusThis study
    −1657As CAI4 but with plasmid p1657 integrated at the ENO1 locusThis study
    −1535As CAI4 but with plasmid p1535 integrated at the ENO1 locusThis study
    HGFP3(−1410)As CAI4 but with plasmid pHWP1GFP3 integrated at the ENO1 locus39
    −1366As CAI4 but with plasmid p1366 integrated at the ENO1 locusThis study
    −1242As CAI4 but with plasmid p1242 integrated at the ENO1 locusThis study
    −1209As CAI4 but with plasmid p1209 integrated at the ENO1 locusThis study
    −1153As CAI4 but with plasmid p1153 integrated at the ENO1 locusThis study
    −1063As CAI4 but with plasmid p1063 integrated at the ENO1 locusThis study
    −831As CAI4 but with plasmid p831 integrated at the ENO1 locusThis study
    −803As CAI4 but with plasmid p803 integrated at the ENO1 locusThis study
    −555As CAI4 but with plasmid p555 integrated at the ENO1 locusThis study
    −140As CAI4 but with plasmid p140 integrated at the ENO1 locusThis study
    EAs CAI4 but with plasmid pEGFP3 integrated at the ENO1 locusThis study
    K2As CAI4 but with plasmid pK2GFP3 integrated at the ENO1 locusThis study
    MAs CAI4 but with plasmid pMGFP3 integrated at the ENO1 locusThis study
    SAs CAI4 but with plasmid pSGFP3 integrated at the ENO1 locusThis study
    HCRENOAs CAI4 but with plasmid pEX-1 integrated at the ENO1 locusThis study
    SENOAs CAI4 but with plasmid pSENO1 integrated at the ENO1 locusThis study
Plasmids
    pCR4-TOPOKanr Ampr lacZα; maintenance of PCR productsInvitrogen, Carlsbad, CA
    pPICZαCZeor; protein expression in Pichia pastorisInvitrogen, Carlsbad, CA
    pBAD202/D-TOPOKanr; protein expression in E. coliInvitrogen, Carlsbad, CA
    pHWP1GFP3pBluescript SK(−) with 1,410 bp upstream of HWP1 driving GFP expression39
    pENO1pBluescript SK(−) with full ENO1 open reading frame39
    pENO1GFP3pBluescript SK(−) with full ENO1 promoter driving GFP expression39
    pBSRI 1.9-16pBluescript SK(−) with 11.9-kb EcoRI C. albicans genomic clone encompassing HWP1 promoterThis study
    pBSBglII1.8pBluescript SK(−) with 1.8-kb BglII C. albicans genomic clone encompassing HWP1 positions −1410-+602039
    p1902.GFP3pBluescript SK(−) with 1,902 bp upstream of HWP1 driving GFP expressionThis study
    pHWP1.2025GFP3pBluescript SK(−) with 2,025 bp upstream of HWP1 driving GFP expressionThis study
    p1782pHWP1GFP3 with HWP1 promoter positions −1782-+60This study
    p1657pHWP1GFP3 with HWP1 promoter positions −1657-+60This study
    p1535pHWP1GFP3 with HWP1 promoter positions −1535-+60This study
    p1366pHWP1GFP3 with HWP1 promoter positions −1366-+60This study
    p1242pHWP1GFP3 with HWP1 promoter positions −1242-+60This study
    p1209pHWP1GFP3 with HWP1 promoter positions −1209-+60This study
    p1153pHWP1GFP3 with HWP1 promoter positions −1153-+60This study
    p1063pHWP1GFP3 with HWP1 promoter positions −1063-+60This study
    p831pHWP1GFP3 with HWP1 promoter positions −831-+60This study
    p803pHWP1GFP3 with HWP1 promoter positions −803-+60This study
    p555pHWP1GFP3 with HWP1 promoter positions −555-+60This study
    p140pHWP1GFP3 with HWP1 promoter positions −140-+60This study
    pEGFP3pHWP1GFP3 with HWP1 promoter positions −1410-−1042, −555-+60This study
    pK2GFP3pHWP1GFP3 with HWP1 promoter positions −1288-−1042, −555-+60This study
    pMGFP3pHWP1GFP3 with HWP1 promoter positions −1902-−1657, −555-+60This study
    pSGFP3pHWP1GFP3 with HWP1 promoter positions −1902-−1410, −1042-+60This study
    pSENO1pSGFP3 with HWP1 promoter positions −1410-−1042 replaced with 376 bp of ENO1 coding regionThis study
    pEX-1pENO1GFP3 with ENO1 promoter replaced by HWP1 promoter −1410-−1042This study
    pSKD19pCR4-TOPO with HWP1 promoter positions −1410-−1162This study
    pSKD306pPICZαC with N-terminal His6-tagged NHP6This study
    pSKD703pBAD202/D-TOPO with N-terminal His6-tagged NHP6This study
    pSKD710pBAD202/D-TOPO with GCF1 without stop codonThis study
    pSKD721pBAD202/D-TOPO with HTB1 without stop codonThis study
    pSKD723pBAD202/D-TOPO with HTA1 without stop codonThis study