TABLE 2.

Ascomycete proteins that have orthologs in D. bruxellensis but not S. cerevisiae

Functional class and NCBI accession no.SpeciesGene nameFunction
Nitrate metabolism
    CAA11229Hansenula polymorphaYNT1Nitrate transporter
    CAA11232Hansenula polymorphaYNR1Nitrate reductase
    CAA11230Hansenula polymorphaYNI1Nitrite reductase
    CAA11231Hansenula polymorphaYNA1Nitrate assimilation pathway-specific Zn(II)2Cys6 transcription factor
    CAC16081Hansenula polymorphaYNA2Nitrate assimilation pathway-specific Zn(II)2Cys6 transcription factor
Metabolism of alternative carbon sources
    AAX31178Candida albicansNAG1Glucosamine-6-phosphate deaminase
    CAH02587Kluyveromyces lactisLAC4β-Galactosidase
    CAE47547Ambrosiozyma monosporaALX1l-Xylulose reductase
    CAG88556aDebaryomyces hanseniiInositol oxygenase
    CAG90181aDebaryomyces hanseniiPhosphatidylinositol-specific phospholipase C
    BAD32688Hansenula polymorphaGlycerol dehydrogenase
    BAD32689Pichia ofunaensisDihydroxyacetone reductase
Transporters
    EAK93787Candida albicansHGT1High-affinity glucose transporter
    AAB17122Debaryomyces occidentalisHAK1High-affinity potassium transporter
    EAL02606Candida albicansNUPPurine nucleoside permease
    EAK96574Candida albicansUAP2Potential purine permease
Lipid metabolism
    BAD11952Saccharomyces kluyveriFAD3ω-3 fatty acid desaturase
    AAU10084Pichia pastorisΔ8-(E)-sphingolipid desaturase
    AAU10085Pichia pastorisΔ4-(E)-sphingolipid desaturase
    AAZ08581Ashbya gossypiiSphingolipid C9-methyltransferase
    AAK73020Pichia pastorisCeramide glucosyltransferase
Respiratory chain complexes
    CAG86996aDebaryomyces hanseniiComplex I 14-kDa subunit
    CAG88782aDebaryomyces hanseniiComplex I 20-kDa subunit
    CAG84577aDebaryomyces hanseniiComplex I 24-kDa subunit
    CAG89146aDebaryomyces hanseniiComplex I 49-kDa subunit
    CAG89064aDebaryomyces hanseniiComplex I 51-kDa subunit
    CAG90271aDebaryomyces hanseniiComplex I 75-kDa subunit
    CAG85893aDebaryomyces hanseniiAOX1SHAM-sensitive alternative terminal oxidase 1
Peroxisome
    CAA82928Hansenula polymorphaPER1Peroxisomal matrix protein
    AAD52811Hansenula polymorphaPEX1Peroxin
    P78723Hansenula polymorphaPEX14Peroxin
    CAA65646Pichia pastorisPEX2Peroxin
    AAF19606Pichia pastorisPEX17Peroxin
    AAD43507Pichia pastorisPEX19Peroxin
Other
    CAG60540aCandida glabrataPRN1Putative pirin
    CAG85416aDebaryomyces hanseniiURA9Dihydroorotate dehydrogenase 2
    CAG86646aDebaryomyces hanseniiBeta-alanine synthase
    CAH00925aKluyveromyces lactisGTP cyclohydrolase II
    CAG80587aYarrowia lipolyticaFAD-dependent oxidoreductase
    BAB12222Candida boidiniiDAO1d-Amino acid oxidase
    CAH02585Kluyveromyces lactisAcid phosphatase
    AAS51137Ashbya gossypiiCalcineurin-like phosphoesterase
    EAL00803Candida albicansPotential secreted Cu/Zn superoxide dismutase
    EAK95140Candida albicansPossible thiamine biosynthesis enzyme
    EAL02572aCandida albicansRas GTPase-activating protein
    CAG85348aDebaryomyces hanseniiPotential histone binding protein
    CAG78240aYarrowia lipolyticaTranslation initiation factor 3 subunit 7
    EAL04857Candida albicansNegative regulator of iron uptake genes
    EAK96905Candida albicansPotential fungal zinc cluster transcription factor
    AAQ75382Hansenula polymorphaSWI1Global transcription activator
  • a For this gene, the best BLAST hit of the D. bruxellensis sequence in the NR database is an unannotated or hypothetical gene, and the functional annotation given here is taken from the highest annotated hit, where the bit score of the annotated hit was at least 90% that of the best hit and sequence comparison indicates that the genes are homologs.