TABLE 1.

Yeast strains used in this study

StrainGenotype
AN120a MAT a /MATα ura3/ura3 his3/his3 leu2/leu2 trp1/trp1 arg4/ARG4 rme1::LEU2/RME1
Y893b MATa/MATα leu2-27/leu2-1 ura3-50/ura3-50 spo13-1/spo13-1 can1-101/can1-101 lys2::ura3ΔNcoI/lys2::ura3ΔNcoI his3Δ/his3Δ his4/his4
Y6582Y893 plus YEp352 (2μm URA3)
Y6583Y893 plus pME2441 (DTR1-GFP 2μm URA3)
Y5120 MATα ura3 his3 leu2 trp1 arg4 rme::LEU2 sso1::HIS3 DTR1-GFP::TRP1
Y7047b MAT a /MATα dtr1::KanMX/dtr1::KanMX leu2-x/leu2-y his4-x/his4-y trp1/trp1 ura3-1/ura3-1 thr1/THR1 lys2/lys2 CYH1/CYH1 ADE2/ade2-1
Y7006AN120, but homozygous MPC54-RFP::URA3
Y7730Y7006 plus pRS424-DTR1-GFP (2μm TRP1)
Y7419AN120 plus pME2599 (dtr1NΔ-GFP 2μm TRP1)
Y7747Y7006 plus pME2599 (dtr1NΔ-GFP 2μm TRP1)
Y7732Y7006 plus pME2611 (dtr1C10Δ-GFP 2μm TRP1)
Y7733Y7006 plus pME2630 (dtr1C15Δ-GFP 2μm TRP1)
Y7735Y7006 plus pME2585 (dtr1T568A-GFP 2μm TRP1)
Y7749Y7006 plus pME2635 (dtr1LA-GFP 2μm TRP1)
Y7784Y7006 plus pME2667 (dtr1LATA-GFP 2μm TRP1)
Y7884Y7006 plus pME2686 (dtr1LA-C10Δ-GFP 2μm TRP1)
Y7479AN120 plus pME2606 (CLB2p-DTR1-GFP 2μm TRP1)
Y7557AN120, but homozygous vps54::KanMX plus pME2606 (CLB2p-DTR1-GFP 2μm TRP1)
Y7592AN120 plus pME2619 (CLB2p-dtr1C10Δ-GFP 2μm TRP1)
Y7777AN120 plus pME2649 (CLB2p-dtr1C15Δ-GFP 2μm TRP1)
Y7807AN120, but homozygous end3::KanMX4 plus pME2649 (CLB2p-dtr1C15Δ-GFP 2μm TRP1)
Y7752AN120 plus pME2653 (CLB2p-dtr1LA-GFP 2μm TRP1)
Y7612AN120 plus pME2620 (CLB2p-dtr1T568A-GFP 2μm TRP1)
Y7803AN120 plus pME2669 (CLB2p-dtr1LAT568A-GFP 2μm TRP1)
Y7889AN120 plus pME2689 (CLB2p-dtr1LA-C10Δ-GFP 2μm TRP1)
Y7657AN120 plus pME2629 (dtr1Loop6Δ-RFP 2μm TRP1) and pCP217 (pRS316 SEC63-GFP CEN URA3)
Y7526AN120 plus pME2611 (dtr1C10Δ-GFP 2μm TRP1)
Y7637AN120 plus pME2630 (dtr1C15Δ-GFP 2μm TRP1)
Y7782AN120 plus pME2626 (DTR1-RFP 2μm TRP1) and pME2665 (dtr1C10Δ-GFPURA3)
Y8206AN120 plus pME2626 (DTR1-RFP 2μm TRP1) and pME2726 (GFP-DTR1 2μm URA3)
Y8207AN120 plus pME2626 (DTR1-RFP 2μm TRP1) and pME2730 (GFP-dtr1C10Δ 2μm URA3)
Y7820AN120, but heterozygous SEC7-dsRED::TRP1 plus pME2665 (dtr1C10Δ-GFP 2μm URA3)
Y7744AN120, but heterozygous SEC7-dsRED::TRP1 plus pME2650 (dtr1C15Δ-GFP 2μm URA3)
Y8018AN120 plus pME2650 (dtr1C15Δ-GFP 2μm URA3) and pME2697 (mRFP-GOS1 2μm TRP1)
Y8044AN120 plus pME2650 (dtr1C15Δ-GFP 2μm URA3) and pME2698 (mRFP-SED5 2μm TRP1)
Y7449AN120, but homozygous VPS10-GFP::KanMX4
Y7451AN120, but homozygous VPS10-CΔ-GFP::KanMX4
Y7750AN120, but homozygous VPS10-CΔ-DTR1C16-GFP::KanMX4
Y8007AN120, but homozygous VPS10-TMΔCΔ-DTR1TMC10-GFP::KanMX4
Y8190AN120, but homozygous VPS10-TMΔCΔ-DTR1TM-GFP::KanMX4
  • a From reference 30.

  • b Derived from S288C.