TABLE 2.

Melanin-related products from extracts of CDYB cultures containing single and combined strains of Wangiella dermatitidisa

Strain(s)bProductse
Wild typeND
Wild type*Flaviolin,c biflaviolin,c 4-HS, 5-HS, 2-HJ, scytalone
WdPks1ND
WdPks1*ND
WdYel23-AF,c flaviolind
WdYel2*3-AF,c flaviolind
Wdyg1Δ-2WdYG1ND
Wdyg1Δ-2WdYG1*Flaviolin,c biflaviolin,c 4-HS, 5-HS, 2-HJ, scytalone
WdYel2 + WdPks14-HS, 5-HS, 2-HJ, scytalone, 4,8-DHT
WdYel2 + WdPks1*Flaviolin,c biflaviolin,c scytalone, 5-HS, 2-HJ, 3-HJ
Wild type + WdPks1ND
Wdyg1Δ-2WdYG1 + WdPks1ND
  • a The cultures were grown for 6 days on a rotary shaker at 250 rpm in the dark at 25°C.

  • b *, Cultures contained 30 μg/ml tricyclazole.

  • c 3-AF, flaviolin, and 3,3′-biflaviolin were the main metabolites present.

  • d Flaviolin was found only occasionally and only in trace amounts.

  • e ND, not detected.