TABLE 4.

Known melanin metabolites obtained after T4HN and 3-AF were added to albino homogenates of Wangiella dermatitidis under anaerobic conditions in the absence and presence of tricyclazolea

SubstrateProduct(s)b
AT4HND2HN*
T4HND2HN*
3-AF4-HS,* 5-HS, 2-HJ,* 3,4,8-THT,* 4,8-DHT,* juglone*
Flaviolin5-HS, 2-HJ,* 3,4,8-THT,* 4,8-DHT,* juglone*
  • a Initial concentration of AT4HN and 3-AF was 1.0 mM; initial concentration of T4HN and flaviolin was 0.5 mM. Reactions were carried out in 10-ml assay systems.

  • b *, product production was blocked in assays containing 30 μg/ml tricyclazole.