TABLE 1.

Primer sequences for RT-PCR of proteinases

OrganismProteinase used for identificationaPrimer sequence
SenseAntisense
A. castellaniiCysteine proteinase5′-CCGAGCAGAACCTCATCGATT5′-CCATGCCCTTGTTGTTGATGAT
Serine-type endopeptidase5′-GAAGGCGCTCACCGAATACAT5′-GGTTCGTCATCTGCTGATAGCC
Peptidase M285′-TCGGTATCGTGACGGACTACGT5′-TAGCCGCACTTGGTGTTCTTG
Aspartyl aminopeptidase-like protein5′-ACCATCGACATCGGTTTGC5′-TGAAGTCGGAGTAGAAGGCCTT
Subtilisin-like serine proteinase5′-CAACAAGATGCTCTACACGCC5′-CGTAATCGGAGCCATCCAACA
18S rDNA5′-TCCAAT TTTCTGCCACCGAA5′-ATCATTACCCTAGTCCTCGCGC
A. healyiSubtilisin-like serine proteinase5′-TCAAGGTGCTCGGATGCAAT5′-ATGTTAGCCACAGACTGCGTC
  • a rDNA, ribosomal DNA.