TABLE 1.

Primers and plasmids used in this study

N. crassa geneSequenceaHost vectorPlasmid
5′ primer3′ primer
cax ggtttctagaCCATGGACTCGGAACGCCGC ccaacccgggGAAAATGGTGGAAAGTTTCTG pMF272pcax-GFP
cax ccttacgcgtATGGACTCGGAACGCCGC ggttagatctGAAAATGGTGGAAAGTTTCTGAAG pMF334pRFP-cax
nca-1 gcattctagaATATGGAGGCGGCGTTTGCAAAGC ccaacccgggGAGATCCTTCTTCTTATCTG pMF272pnca-1-GFP
nca-1 ctttacgcgtATGGAGGCGGCGTTTGCAAAG gtcttctagagGAGATCCTTCTTCTTATCTGAGGACG pMF334pRFP-nca-1
nca-2 cctatctagaCCATGGACCCCATTGCCAGTC ccaacccgggGGCCGACTTTTGCCCTGG pMF272pnca-2-GFP
nca-2 ccttacgcgtATGGACCCCATTGCCAGTCAC ggttagatctGGCCGACTTTTGCCCTGG pMF334pRFP-nca-2
nca-3 cctctctagaGCTAATGGCGGACCATGAGCCG cgaacccgggAGACCAACTCCTCCCCAACC pMF272pnca-3-GFP
pmr cctgtctagaCCATCATGGACCGACTCAGC cataggatcccTACAGCCTGGCTATAGTTAAC pMF272ppmr-GFP
pmr ctttggcgcgcctATGGACCGACTCAGCGCGTG cccctctagagTACAGCCTGGCTATAGTTAACACC pMF334pRFP-pmr
grp-78 cctttctagaGTAATGGAGCACCGAACGCGAAG gttttcccgggAAGCTCGTCATGGCCAGCAG pMF272pgrp-GFP
grp-78 ccttacgcgtATGGAGCACCGAACGCGAAG ggttagatctAAGCTCGTCATGGCCAGCAG pMF334pRFP-grp
dpm cctttctagaCACCATGGCTCCCACCAAG gttttcccgggCGTGGACCACCAAAGCTGC pMF272pdpm-GFP
dpm ccttacgcgtATGGCTCCCACCAAGACCAC ggttagatctCGTGGACCACCAAAGCTGC pMF334pRFP-dpm
vps-52 cctttctagaCGCTATGTGGCTTGATCGACGTC gttttcccgggGATATTCTCTTGTACGCTCGCC pMF272pvps-52-GFP
vps-52 ccttacgcgtATGTGGCTTGATCGACGTC ggttagatctGATATTCTCTTGTACGCTCGCC pMF334pRFP-vps-52
vam-3 ccttacgcgtATGTCATTCGATCAGCTCTCC ggttagatctGCCCAAGAACACCGCCAAC pMF334pRFP-vam-3
vma-1 cacaggcgcgcctATGGCTCCCGTAAGCCTTG gttttggccggccCTCATCAATGACAGACGCGAAC pMF334pRFP-vma-1
vma-5 cacatctagaGACCATGGCACCGACAACTC ctctcccgggAGGAAATTCAAATTCATAATACAC pMF272pvma-5-GFP
arg-4 ccctacgcgtATGGAGAGCCTCAACGGCTG cctctctagagCGTGCGGTAGTCTCCATTAATGG pMF272parg-4-GFP
  • a The region in uppercase letters is the sequence in the N. crassa gene. The region in lowercase letters contains the restriction site used for construction of the plasmid.