TABLE 3.

Plasmids used in this study

NameDescriptionSource
pACTII LEU2, 2μm two-hybrid vector with GAL4 activation domain 18
PBTM116-AFR1plexA-DBD-AFR1 38
PBTM116-afr1474plexA-DBD-afr1474 25
pJB20pBTM116-afr1VA/FAThis study
pHH148 GLC7 in pACTIIThis study
pCDV471 GLC7 in pEG202 (ADH1-LexA-DBD) 41
pAR16pGADC1-BNI4 39
pJK52YEplac181-AFR1 25
YEp181-afr1-C7YEplac181-afr1474-620 25
pRS425 LEU2, 2μm vector 10