TABLE 4.

List of genes for generation of insertion mutant strains

ORF19 no.Gene namePossible functionFold change bya:TIGR insertion IDInsertion mutationHyphaeHost cell damage (% of wild type ± SD)bProtamine susceptibility
MicroarrayReal-time PCR
EpithelialEndothleialEpithelialEndothelialEpithelialEndothelial
orf19.348Uncharacterized2.21.02.5NDcCAGOO23No
orf19.1077ATM1Member of MDR subfamily of ABC family2.21.13.5NDCAGQD15YesNormal91 ± 5NDNormal
orf19.4656Uncharacterized2.2−1.13.8NDCAGPD46YesNormal112 ± 4NDNormal
orf19.4793Uncharacterized2.0−1.11.6NDCAGAQ76YesNormal111 ± 15NDNormal
orf19.6266Uncharacterized, CCR4-NOT core complex2.0−1.12.5NDCAGB385No
orf19.2398Uncharacterized2.11.22.5NDCAGN836YesNormal114 ± 8NDNormal
orf19.6931CLP1Uncharacterized, probable cleavage/polyadenylation factor2.0d−1.51.9NDCAGI909YesNormal109 ± 24NDNormal
orf19.200THO1Uncharacterized2.1e−1.1e2.5eNDCAGDN85No
orf19.4655OPT6Putative oligopeptide transporter2.4e−1.7e3.1eNDCAGFI33YesNormal94 ± 9NDNormal
orf19.7001YCK2Casein kinase I (by homology)2.2e−1.4e3.5e2.0eCAGDI41YesShort/multiple48 ± 879 ± 8Increased
orf19.202CDC47Cell division control protein2.5e−1.5e1.6eNDCAGCJ23No
orf19.1504Uncharacterized, putative patatin-like phospholipase2.61.14.3NDCAGBR73YesNormal107 ± 13NDNormal
orf19.3455LPE10Uncharacterized, putative magnesium transporter activity2.72.52.6NDCAGDF12No
orf19.4922Uncharacterized2.31.01.6NDCAGF844YesNormal122 ± 10NDNormal
orf19.3260SIS2Uncharacterized−1.22.1dND3.0dCAGB279No
orf19.5568VPS51Protein targeting to vacuole (by homology)1.2d2.1d2.2d2.9dCAGDP76YesShort2 ± 133 ± 4Increased
orf19.6168Uncharacterized, possible structural constituent of cytoskeleton1.22.3ND2.4CAGEP09YesNormalND97 ± 9Normal
orf19.1766IFO3Uncharacterized, similar to Streptomyces coelicolor putative hydrolase1.32.1ND1.8CAGL436YesNormalND80 ± 3Normal
orf19.3740PGA23Putative glycosylphosphatidylinositol-anchored protein with unknown function1.0d2.7dND2.8dCAGN829YesNormalND99 ± 2Normal
orf19.4142Uncharacterized1.0d2.4dND1.6dCAGC641YesNormalND71 ± 16Normal
orf19.4791Uncharacterized−1.8d2.0dND2.2dCAGE249YesNormalND107 ± 9Normal
orf19.4646UEC1Uncharacterized1.22.01.13.4CAGLQ62YesThickened36 ± 1891 ± 5Increased
orf19.3664HSP31Heat shock protein (by homology)−1.82.2ND4.9CAGJM81YesNormalND84 ± 13Normal
orf19.4894Uncharacterized−1.5d3.4dND2.3dCAGKC82YesNormalND73 ± 11Normal
orf19.1980GIT4Putative glycerophosphoinositol permease1.1d2.5dND1.7dCAGP819YesNormalND77 ± 12Normal
orf19.1939Uncharacterized−1.1d2.1dND1.5dCAGM083YesNormalND67 ± 6Normal
orf19.6392Uncharacterized−1.1d2.3dND1.5dCAGJP50YesNormalND96 ± 16Normal
orf19.403PCL2Cyclin-dependent protein kinase−1.3d2.3dND2.0dfYesNormalND94 ± 6Normal
orf19.1148CIS312Uncharacterized1.02.4ND2.5fYesNormalND96 ± 6Normal
  • a After 45 min of incubation unless otherwise specified.

  • b The wild-type control strain was DAY185.

  • c ND, not determined.

  • d After 90 min of incubation.

  • e After 180 min of incubation.

  • f —, insertion cassettes for orf19.403 and orf19.1148 were made in the present study.