TABLE 1.

Yeast strains used in this study

StrainGenotypeaSource or reference
NJ1 (wild type)h+ his7-366 ade6-M210Laboratory stock
NJ3h+ rav1::kanr his7-366 ade6-M210This study
NJ4h+ lag1::kanr his7-366 ade6-M210This study
NJ5h lac1::kanr his7-366 ade6-M210This study
NJ13h+ rav1-13myc:kanrhis7-366 ade6-M210This study
NJ88h? rav1::kanrlag1::kanr his7-366 ade6This study
NJ89h? rav1::kanrlac1::kanr his7-366 ade6This study
NJ168h+ vma2::kanr his7-366 ade6-M210This study
NJ490h kanr-p3nmt1-3HA-skp1+ his7-366 ade615
NJ838h+ rav1-1:13myc:kanr his7-366 ade6-M210This study
NJ844h+ rav2::natr his7-366 ade6-M210This study
NJ864h kanr-p3nmt1-3HA-skp1+ his7-366 ade6rav1-13myc:kanrThis study
NJ865h+ rav2-13myc his7-366 ade6-M210This study
NJ883h vma1::ura416
NJ885h vma3::ura4 ura4-D18
NJ887h? vma1::ura4 rav1::kanr his7-366 ade6This study
NJ1029h? rav2-13myc his7-366 ade6-M210 rav1::kanrThis study
  • a All S. pombe strains are leu1-32 ura4-D18, except NJ885 and NJ887, which have the ura4-C190T allele instead of ura4-D18.