TABLE 2.

Plasmids used in this study

PlasmidDescription and/or relevant genotypeReference or source
Plasmids for cloning, construction, and marker removal
    pGEM-TCloning vector; Kmr AprPromega
    pAP599Cloning vector with 2 FRT direct repeats flanking the hygromycin marker [FRT-PPGK::hph::(3′ UTR of HIS3)-FRT] for construction of multiple mutants; URA3 Hygr Ampr19
    pMZ17Replicative vector expressing ScFLP1 (recombinase gene) for removing the hygromycin marker; PPGK::FLP1::(3′ UTR of HIS3) CgCEN ARS AmprCormack lab collection
    pBC34.1pUC19::CgURA3; 2.2-kb PstI fragment; Apr15
Plasmids for deletion
    pCV15 cta1ΔA 0.906-kb SpeI/BamHI PCR fragment (corresponding to primers 57 and 58) carrying the promoter region of CTA1 and a 0.682-kb HindIII/KpnI PCR fragment (corresponding to primers 60 and 61) carrying the 3′ UTR of CTA1 were cloned into pAP599 AprThis work
    pCV17 yap1ΔA 0.846-kb XhoI/HindIII PCR fragment (corresponding to primers 7 and 8) carrying the promoter region of YAP1 and a 0.652-kb BamHI/SacI PCR fragment (corresponding to primers 11 and 12) carrying the 3′ UTR of YAP1 were cloned into pAP599 AprThis work
    CV21 skn7ΔA 0.875-kb KpnI/XhoI PCR fragment (corresponding to primers 4 and 5) carrying the promoter region of SKN7 and a 0.929-kb BamHI/SacI PCR fragment (corresponding to primers 1 and 2) carrying the 3′ UTR of SKN7 were cloned into pAP599 AprThis work
    pRD96 msn2ΔA 0.691-kb KpnI/XhoI PCR fragment (corresponding to primers 2984 and 2985) carrying the promoter region of MSN2 and a 0.520-kb BamHI/SacI PCR fragment (corresponding to primers 2986 and 2987) carrying the 3′ UTR of MSN2 were cloned into pAP599 AprR. Domergue and B. Cormack
    pRD97 msn4ΔA 0.564-kb KpnI/XhoI PCR fragment (corresponding to primers 2990 and 2991) carrying the promoter region of MSN4 and a 0.535-kb BamHI/SacI PCR fragment (corresponding to primers 2992 and 2993) carrying the 3′ UTR of MSN4 were cloned into pAP599 AprR. Domergue and B. Cormack