TABLE 1.

Cell density and capsule thickness in continuous and diluted cultures

HourContinuous cultureDiluted culture
Cell densityaCapsule thicknessbCell densityCapsule thickness
119.1 × 1070.80 ± 0.039.1 × 1070.80 ± 0.03
241.5 × 1081.35 ± 0.068.6 × 1070.90 ± 0.02
351.8 × 1081.37 ± 0.076.4 × 1070.82 ± 0.03
482.1 × 1081.60 ± 0.191.0 × 1081.02 ± 0.05
754.3 × 1081.54 ± 0.081.7 × 1080.93 ± 0.02
  • a Cell density at the indicated time of growth (cells/ml), determined by counting cells on a hemocytometer.

  • b Capsule thickness of India-ink-stained cells, measured using an Axioskop 2 fluorescence microscope (Carl Zeiss). The averages ± standard errors of the means (μm) of 50 cells for each time point are tabulated.